Peng JianZong, Lai LiuJing, Wang XiaoJing
College of Life Sciences, South China Normal University, Guangdong Provincial Key Lab of Biotechnology for Plant Development, Guangzhou 510631, China.
Sci China C Life Sci. 2008 Jun;51(6):520-5. doi: 10.1007/s11427-008-0067-z. Epub 2008 May 17.
PRPs (proline-rich proteins) are a group of cell wall proteins characterized by their proline and hydroproline-rich repetitive peptides. The expression of PRPs in plants is stimulated by wounding and environmental stress. GASA (gibberellic acid stimulated in Arabidopsis) proteins are small peptides sharing a 60 amino acid conserved C-terminal domain containing twelve invariant cysteine residues. Most of GASAs reported are localized to apoplasm or cell wall and their expression was regulated by gibberellins (GAs). It has been reported that, in French bean, these two proteins encoding by two distinct genes formed a two-component chitin-receptor involved in plant-pathogen interactions when plant was infected. We cloned a full-length cDNA of PRGL (proline-rich GASA-like) gene which encodes a protein containing both PRP and GASA-like domains. It is demonstrated that PRGL is a new protein with characteristics of PRP and GASA by analyzing its protein structure and gene expression.
富含脯氨酸蛋白（PRPs）是一类细胞壁蛋白，其特征在于富含脯氨酸和羟脯氨酸的重复肽段。植物中PRPs的表达受创伤和环境胁迫的刺激。GASA（拟南芥中受赤霉素刺激）蛋白是一类小肽，共享一个包含12个不变半胱氨酸残基的60个氨基酸的保守C末端结构域。报道的大多数GASAs定位于质外体或细胞壁，其表达受赤霉素（GAs）调控。据报道，在菜豆中，当植物被感染时，这两个由不同基因编码的蛋白形成了一个参与植物-病原体相互作用的双组分几丁质受体。我们克隆了一个PRGL（富含脯氨酸的GASA样）基因的全长cDNA，该基因编码一种同时包含PRP和GASA样结构域的蛋白。通过分析其蛋白质结构和基因表达，证明PRGL是一种具有PRP和GASA特征的新蛋白。
