Hendricks G L, Siegel H S, Mashaly M M
Department of Poultry Science, Pennsylvania State University, University Park 16802.
Proc Soc Exp Biol Med. 1991 Apr;196(4):390-5. doi: 10.3181/00379727-196-43204.
Treatment of splenic leukocytes from Cornell K strain male chickens (homozygous at the B15 locus of the major histocompatibility complex) with ovine corticotropin-releasing factor (oCRF), before their co-incubation with naive chicken adrenal cells, resulted in an increase in corticosterone production. Supernatants from the oCRF-treated splenic leukocytes caused a time-dependent increase in corticosterone production when incubated with chicken adrenal cells. Adding oCRF directly to chicken adrenal cells did not increase corticosterone production. Pretreatment of peripheral leukocytes with oCRF increased their activity in a concanavalin A mitogen assay. Thus, chicken leukocytes stimulated with corticotropin releasing factor appear to increase the production of an "adrenocorticotropin-like" substance (adrenocorticotropin-like because it increases corticosterone production by adrenal cells), and increased their cell-mediated immune activity.
用绵羊促肾上腺皮质激素释放因子（oCRF）处理来自康奈尔K系雄性鸡（在主要组织相容性复合体的B15位点纯合）的脾白细胞，然后将其与未接触过抗原的鸡肾上腺细胞共同孵育，结果皮质酮产量增加。用oCRF处理过的脾白细胞的上清液与鸡肾上腺细胞孵育时，会导致皮质酮产量随时间增加。直接向鸡肾上腺细胞中添加oCRF不会增加皮质酮产量。用oCRF预处理外周白细胞会增加其在伴刀豆球蛋白A丝裂原试验中的活性。因此，用促肾上腺皮质激素释放因子刺激的鸡白细胞似乎会增加一种“促肾上腺皮质激素样”物质的产生（促肾上腺皮质激素样是因为它会增加肾上腺细胞的皮质酮产量），并增强其细胞介导的免疫活性。
