Hendricks G L, Mashaly M M, Siegel H S
Department of Poultry Science, Pennsylvania State University, University Park 16802, USA.
Poult Sci. 1995 Feb;74(2):337-42. doi: 10.3382/ps.0740337.
A RIA for mammalian adrenocorticotropic hormone (ACTH) was modified and validated to measure chicken ACTH. The assay was capable of detecting an increase in chicken plasma ACTH following treatments known to increase plasma ACTH. Both splenic and peripheral blood leukocytes stimulated with corticotropin-releasing factor (CRF) showed a significant increase in ACTH production compared with unstimulated leukocytes. This finding supports the conclusion that the substance produced by leukocytes previously shown in our laboratory to stimulate adrenal cells to secrete corticosterone is immunoreactive ACTH.
一种用于检测哺乳动物促肾上腺皮质激素（ACTH）的放射免疫分析方法经过修改和验证，用于测量鸡的促肾上腺皮质激素。该检测方法能够检测出已知可增加血浆促肾上腺皮质激素的处理后鸡血浆促肾上腺皮质激素的增加。与未刺激的白细胞相比，用促肾上腺皮质激素释放因子（CRF）刺激的脾脏和外周血白细胞的促肾上腺皮质激素产生量均显著增加。这一发现支持了以下结论：我们实验室之前证明能刺激肾上腺细胞分泌皮质酮的白细胞产生的物质是免疫反应性促肾上腺皮质激素。
