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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Hendricks G L, Mashaly M M

Department of Poultry Science, Pennsylvania State University, University Park 16802-3501, USA.

Br Poult Sci. 1998 Mar;39(1):123-7. doi: 10.1080/00071669889493.

Corticotropin-releasing factor (CRF), a neuropeptide with immunomodulating properties, is known to stimulate avian splenic leukocytes to produce adrenocorticotropic hormone (ACTH). 2. The present study was to determine which avian splenic leukocyte subpopulation(s) produce ACTH in response to CRF stimulation. 3. Splenic leukocytes from 8-week-old male chickens were isolated on Histopaque 1077 and macrophages were separated from lymphocytes by adherence to a polystyrene surface. 4. Different concentrations of CRF (0, 5, 50, 500 or 1000 ng/m) were incubated with the different leukocyte populations, supernatants were collected and ACTH was measured using a radioimmunoassay. 5. Isolated macrophages, stimulated with CRF, produced significantly more ACTH than either unstimulated macrophages or CRF-stimulated lymphocytes, suggesting that ACTH may be produced by a particular subset of leukocytes, the macrophages (and monocytes), in response to CRF stimulation.

促肾上腺皮质激素释放因子（CRF）是一种具有免疫调节特性的神经肽，已知它能刺激禽类脾脏白细胞产生促肾上腺皮质激素（ACTH）。2. 本研究旨在确定哪些禽类脾脏白细胞亚群在CRF刺激下会产生ACTH。3. 从8周龄雄性鸡的脾脏中分离白细胞，利用Histopaque 1077进行分离，通过贴壁于聚苯乙烯表面将巨噬细胞与淋巴细胞分离。4. 将不同浓度的CRF（0、5、50、500或1000 ng/m）与不同的白细胞群体一起孵育，收集上清液并使用放射免疫测定法测量ACTH。5. 经CRF刺激的分离巨噬细胞产生的ACTH明显多于未刺激的巨噬细胞或经CRF刺激的淋巴细胞，这表明ACTH可能是由特定的白细胞亚群，即巨噬细胞（和单核细胞），在CRF刺激下产生的。

Hendricks G L, Mashaly M M

Department of Poultry Science, Pennsylvania State University, University Park 16802-3501, USA.

Br Poult Sci. 1998 Mar;39(1):123-7. doi: 10.1080/00071669889493.

Corticotropin-releasing factor (CRF), a neuropeptide with immunomodulating properties, is known to stimulate avian splenic leukocytes to produce adrenocorticotropic hormone (ACTH). 2. The present study was to determine which avian splenic leukocyte subpopulation(s) produce ACTH in response to CRF stimulation. 3. Splenic leukocytes from 8-week-old male chickens were isolated on Histopaque 1077 and macrophages were separated from lymphocytes by adherence to a polystyrene surface. 4. Different concentrations of CRF (0, 5, 50, 500 or 1000 ng/m) were incubated with the different leukocyte populations, supernatants were collected and ACTH was measured using a radioimmunoassay. 5. Isolated macrophages, stimulated with CRF, produced significantly more ACTH than either unstimulated macrophages or CRF-stimulated lymphocytes, suggesting that ACTH may be produced by a particular subset of leukocytes, the macrophages (and monocytes), in response to CRF stimulation.

促肾上腺皮质激素释放因子（CRF）是一种具有免疫调节特性的神经肽，已知它能刺激禽类脾脏白细胞产生促肾上腺皮质激素（ACTH）。2. 本研究旨在确定哪些禽类脾脏白细胞亚群在CRF刺激下会产生ACTH。3. 从8周龄雄性鸡的脾脏中分离白细胞，利用Histopaque 1077进行分离，通过贴壁于聚苯乙烯表面将巨噬细胞与淋巴细胞分离。4. 将不同浓度的CRF（0、5、50、500或1000 ng/m）与不同的白细胞群体一起孵育，收集上清液并使用放射免疫测定法测量ACTH。5. 经CRF刺激的分离巨噬细胞产生的ACTH明显多于未刺激的巨噬细胞或经CRF刺激的淋巴细胞，这表明ACTH可能是由特定的白细胞亚群，即巨噬细胞（和单核细胞），在CRF刺激下产生的。