Mechanisms of action involved in the chemoattractant activity of three beta-lactamic antibiotics upon human neutrophils.

The effects produced by three beta-lactamic antibiotics (N-formimidoyl thienamycin or imipenem, cefmetazole and cefoxitin) in vitro on protein carboxylmethylation, cAMP and cGMP levels in human polymorphonuclear neutrophils were studied. These antibiotics (50 micrograms/mL) exhibited chemoattractant activity for phagocytic cells and produced a fast (0.5 min) and significant stimulation of protein carboxylmethylation. They also increased intraphagocytic cGMP levels although no changes in cAMP levels were observed. Since the involvement of the above-mentioned mechanisms in leukotaxis have been established, the stimulation of neutrophil chemotaxis by the three antibiotics studied could possibly be mediated by one or more of these mechanisms.