Rodriguez A B, Barriga C, De la Fuente M
Department of Physiology, Faculty of Science, University of Extremadura, Badajoz, Spain.
Comp Immunol Microbiol Infect Dis. 1993 Jan;16(1):37-50. doi: 10.1016/0147-9571(93)90059-e.
The effects in vitro of Cefoxitin, a beta-lactam antibiotic, at a concentration of 50 micrograms/ml (similar to a therapeutic level in plasma) on each step of the phagocytic process, in addition to antibody-dependent cellular cytotoxicity (ADCC) of blood peripheral human neutrophils have been studied. The antibiotic produced a significant stimulation of adherence capacity, chemotaxis, attachment of Candida albicans to neutrophils and phagocytosis of Candida albicans (with serum, decomplemented serum or without serum), ingestion of inert particles (latex beads), microbicidal activity, and digestion capacity of the ingested material [measured by nitroblue tetrazolium (NBT) reduction]. Moreover, cefoxitin (50 micrograms/ml) demonstrated chemoattractant activity for neutrophils. ADCC was also increased by this antimicrobial agent. These results therefore suggest that a therapeutic concentration of cefoxitin may enhance microbicidal and cytotoxic functions of a pivotal human phagocytic cell: the neutrophil.
已研究了β-内酰胺类抗生素头孢西丁在浓度为50微克/毫升（类似于血浆中的治疗水平）时对吞噬过程各步骤的体外作用，此外还研究了其对人外周血中性粒细胞抗体依赖性细胞毒性（ADCC）的影响。该抗生素显著刺激了黏附能力、趋化性、白色念珠菌与中性粒细胞的附着以及白色念珠菌的吞噬作用（使用血清、补体灭活血清或无血清）、惰性颗粒（乳胶珠）的摄取、杀菌活性以及摄取物质的消化能力[通过硝基蓝四氮唑（NBT）还原测定]。此外，头孢西丁（50微克/毫升）对中性粒细胞表现出趋化活性。这种抗菌剂也增强了ADCC。因此，这些结果表明，头孢西丁的治疗浓度可能增强人体关键吞噬细胞——中性粒细胞的杀菌和细胞毒性功能。
