Saemundsen A K, Purtilo D T, Sakamoto K, Sullivan J L, Synnerholm A C, Hanto D, Simmons R, Anvret M, Collins R, Klein G
Cancer Res. 1981 Nov;41(11 Pt 1):4237-42.
Tissues from patients thought to have Epstein-Barr virus (EBV)-induced lymphoproliferative diseases were probed for EBV genomes using 2 independent hybridization techniques. Tissues from six patients with the X-linked lymphoproliferative syndrome, all five renal allograft recipients with immunoblastic sarcoma, and eight patients with diverse types of immunodeficiency and lymphoproliferative diseases such as fatal infectious mononucleosis or malignant lymphoma associated with antecedent immunodeficiency contained significant numbers of EBV genome equivalents per cell. The use of 2 hybridization probes is recommended to confirm the presence of EBV genomes. The finding of significant numbers of EBV genomes in tissues from patients with immunodeficiency suggests that EBV is the etiological agent of the associated lymphoproliferative diseases.
使用两种独立的杂交技术，对疑似患有爱泼斯坦-巴尔病毒（EBV）诱导的淋巴增殖性疾病患者的组织进行EBV基因组检测。来自6名患有X连锁淋巴增殖综合征的患者、所有5名患有免疫母细胞肉瘤的肾移植受者以及8名患有各种类型免疫缺陷和淋巴增殖性疾病（如致命传染性单核细胞增多症或与先前免疫缺陷相关的恶性淋巴瘤）的患者的组织，每个细胞中都含有大量的EBV基因组当量。建议使用两种杂交探针来确认EBV基因组的存在。在免疫缺陷患者的组织中发现大量EBV基因组，这表明EBV是相关淋巴增殖性疾病的病原体。
