Redondo M, Ruiz-Cabello F, Concha A, Cabrera T, Pérez-Ayala M, Oliva M R, Garrido F
Servicio de Analisis Clínicos e Immunología, Hospital Virgen de las Nieves, Universidad de Granada, Spain.
Cancer Res. 1991 May 1;51(9):2463-8.
We studied the expression of major histocompatibility complex class I antigens in 59 bronchogenic carcinomas, as well as in pneumocytes and epithelial respiratory cells distant from the tumor. We observed in all cases that normal lung tissue expressed major histocompatibility complex class I antigens, while this expression was completely lost in 16 tumors (27%). The defect in HLA gene expression affected both heavy chain and beta 2-microglobulin, as demonstrated by the null reactivity with the monoclonal antibodies GRH1, W6/32, and HC10. Selective underexpression was detected in 1 tumor for HLA-A locus antigens and in 3 tumors for HLA-B locus antigens. Southern blot analyses of major histocompatibility complex class I genes were performed in 20 tumor tissue specimens and 6 cell lines. No class I gene rearrangements were detected using HLA coding and locus specific noncoding probes. We also used the Southern blot method to investigate the possible relationship between c-myc amplification and HLA class I antigens in non-small cell lung cancers and detected no apparent amplification in 20 tumor tissue specimens (5 negative for HLA class I antigens) and 6 cell lines (3 with decreased expression). Northern blot analysis revealed no relationship between c-myc mRNA levels and specific mRNA for HLA-A and HLA-B antigens in cell lines with imbalanced HLA-A or HLA-B expression.
我们研究了59例支气管源性癌组织以及远离肿瘤的肺细胞和呼吸道上皮细胞中主要组织相容性复合体I类抗原的表达情况。我们在所有病例中观察到，正常肺组织表达主要组织相容性复合体I类抗原，而在16例肿瘤（27%）中这种表达完全丧失。HLA基因表达缺陷影响重链和β2-微球蛋白，这通过与单克隆抗体GRH1、W6/32和HC10的无反应性得以证明。在1例肿瘤中检测到HLA-A位点抗原选择性低表达，在3例肿瘤中检测到HLA-B位点抗原选择性低表达。对20个肿瘤组织标本和6个细胞系进行了主要组织相容性复合体I类基因的Southern印迹分析。使用HLA编码和位点特异性非编码探针未检测到I类基因重排。我们还使用Southern印迹法研究了非小细胞肺癌中c-myc扩增与HLA I类抗原之间的可能关系，在20个肿瘤组织标本（5个HLA I类抗原阴性）和6个细胞系（3个表达降低）中未检测到明显扩增。Northern印迹分析显示，在HLA-A或HLA-B表达失衡的细胞系中，c-myc mRNA水平与HLA-A和HLA-B抗原的特异性mRNA之间无相关性。
