Zheng Xi, Cui Xiao-Xing, Huang Mou-Tuan, Liu Yue, Shih Weichung Joe, Lin Yong, Lu Yao Ping, Wagner George C, Conney Allan H
Susan Lehman Cullman Laboratory for Cancer Research, Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA.
Oncol Rep. 2008 Jun;19(6):1583-8.
In the present study, we investigated the effect of voluntary exercise on the formation and growth of the human pancreas Panc-1 and prostate PC-3 tumors in immunodeficient mice. Female severe combined immunodeficient (SCID) mice were injected subcutaneously with human pancreatic cancer Panc-1 cells, and male SCID mice were injected subcutaneously with human prostate cancer PC-3 cells. Voluntary running wheel exercise for 63 days, starting one week before the subcutaneous injection of Panc-1 or PC-3 tumor cells into SCID mice, suppressed the growth of Panc-1 and PC-3 tumors. The exercise regimen increased the food and fluid consumption in the female and male mice. Exercise also decreased the size of the parametrial fat pads in the female mice and the paradidymis fat pads in the male mice, but there was no effect on the body weight. Mechanistic studies showed that voluntary running wheel exercise inhibited proliferation as reflected by a decreased mitosis, and the exercise regimen also stimulated apoptosis as reflected by the increased caspase-3 (active form) expression in the Panc-1 and PC-3 tumors. Voluntary running wheel exercise decreased the ratio of the percent mitotic cells/apoptotic cells in Panc-1 and PC-3 tumors by 38 and 32%, respectively. The present study demonstrated an inhibitory effect of voluntary exercise on the growth of pancreas and prostate tumors in a SCID mouse xenograft model.
在本研究中，我们调查了自愿运动对免疫缺陷小鼠体内人胰腺Panc-1肿瘤和前列腺PC-3肿瘤形成及生长的影响。雌性严重联合免疫缺陷（SCID）小鼠皮下注射人胰腺癌Panc-1细胞，雄性SCID小鼠皮下注射人前列腺癌PC-3细胞。在将Panc-1或PC-3肿瘤细胞皮下注射到SCID小鼠体内前一周开始，进行为期63天的自愿转轮运动，可抑制Panc-1和PC-3肿瘤的生长。该运动方案增加了雌性和雄性小鼠的食物和液体摄入量。运动还减小了雌性小鼠的子宫旁脂肪垫大小以及雄性小鼠的附睾旁脂肪垫大小，但对体重没有影响。机制研究表明，自愿转轮运动抑制了增殖，表现为有丝分裂减少，并且该运动方案还刺激了凋亡，表现为Panc-1和PC-3肿瘤中caspase-3（活性形式）表达增加。自愿转轮运动使Panc-1和PC-3肿瘤中有丝分裂细胞百分比/凋亡细胞百分比的比率分别降低了38%和32%。本研究证明了在SCID小鼠异种移植模型中，自愿运动对胰腺和前列腺肿瘤生长具有抑制作用。
