Williamson A L, Jaskiesicz K, Gunning A
Department of Medical Microbiology, University of Cape Town, Observatory, South Africa.
Anticancer Res. 1991 Jan-Feb;11(1):263-5.
The polymerase chain reaction, with nested degenerate primers which amplify a specific fragment of the L1 gene of wide range of human papillomaviruses, was used to determine whether HPV DNA was present in biopsies from various oesophageal lesions. Ten out of 14 (71%) patients with oesophageal carcinoma were positive for HPV DNA either in the tumour biopsies (6/14) or in adjacent tissue (6/9). HPV DNA was detected in 6 of the 41 biopsies (15%) from patients without oesophageal malignancy. Three of these 6 positive were from biopsies with clear cell acanthosis.
采用聚合酶链反应，利用巢式简并引物扩增多种人乳头瘤病毒L1基因的特定片段，以确定各种食管病变活检组织中是否存在人乳头瘤病毒DNA。14例食管癌患者中有10例（71%），其肿瘤活检组织（6/14）或相邻组织（6/9）中人乳头瘤病毒DNA呈阳性。在41例无食管恶性肿瘤患者的活检组织中，有6例（15%）检测到人乳头瘤病毒DNA。这6例阳性活检组织中有3例来自透明细胞棘皮症活检。
