Meier B, Cross A R, Hancock J T, Kaup F J, Jones O T
Chemisches Institut, Tierärztliche Hochschule, Hannover, Federal Republic of Germany.
Biochem J. 1991 Apr 1;275 ( Pt 1)(Pt 1):241-5. doi: 10.1042/bj2750241.
Human fibroblasts have the capacity to release superoxide radicals upon stimulation of an electron transport system similar to the NADPH oxidase of leukocytes. Two components of the NADPH oxidase system, (1) a flavoprotein of 45 kDa which binds diphenylene iodonium (a compound described as a specific inhibitor of the leukocyte NADPH oxidase), and (2) a low-potential cytochrome b, are present in fibroblast membranes. Fibroblasts exhibit these compounds at lower concentrations than do polymorphonuclear leukocytes or B-lymphocytes. The superoxide-generating system is rather uniformly associated with the outer cell membrane, as shown by light and electron microscopy. Superoxide release upon stimulation with various agents was prevented by the addition of micromolar concentrations of diphenylene iodonium, making an NADPH oxidase a likely source.
人类成纤维细胞在受到类似于白细胞NADPH氧化酶的电子传递系统刺激时，有释放超氧自由基的能力。NADPH氧化酶系统的两个组分，（1）一种45 kDa的黄素蛋白，它能结合二亚苯基碘鎓（一种被描述为白细胞NADPH氧化酶特异性抑制剂的化合物），以及（2）一种低电位细胞色素b，存在于成纤维细胞膜中。成纤维细胞中这些化合物的浓度低于多形核白细胞或B淋巴细胞。如光学显微镜和电子显微镜所示，超氧生成系统相当均匀地与细胞膜外层相关联。加入微摩尔浓度的二亚苯基碘鎓可阻止各种试剂刺激后的超氧释放，这使得NADPH氧化酶可能是超氧的来源。
