Cross A R, Jones O T
Biochem J. 1986 Jul 1;237(1):111-6. doi: 10.1042/bj2370111.
NADPH-dependent superoxide production by the solubilized oxidase of neutrophils was inhibited 36% by diphenylene iodonium at a 1:1 stoichiometry with the enzyme flavoprotein content. Addition of diphenylene iodonium strongly inhibited the NADPH-dependent reduction of both FAD and cytochrome b-245 in steady-state kinetic experiments. Incubation of solubilized enzyme with diphenylene [125I]iodonium resulted in the specific labelling of a polypeptide of Mr 45,000. In the presence of NADPH the amount of label incorporated into the polypeptide was reduced. There was no difference in labelling between enzyme prepared from stimulated or unstimulated cells.
中性粒细胞可溶性氧化酶依赖NADPH的超氧化物生成，在与酶黄素蛋白含量呈1:1化学计量比时，被二苯碘鎓抑制了36%。在稳态动力学实验中，添加二苯碘鎓强烈抑制了FAD和细胞色素b - 245的NADPH依赖性还原。将可溶性酶与二苯[125I]碘鎓一起孵育，导致一种分子量为45,000的多肽被特异性标记。在有NADPH存在的情况下，掺入该多肽的标记量减少。从受刺激或未受刺激细胞制备的酶在标记上没有差异。
