College of Animal Science, Zhejiang University, Hangzhou, China.
J Anim Sci. 2012 Aug;90(8):2671-6. doi: 10.2527/jas.2011-4699. Epub 2012 Jul 10.
A total of 180 weanling pigs (21 ± 3 d of age; 5.98 ± 0.04 kg) were used to investigate the effect of chito-oligosaccharide (COS) on growth performance, intestinal barrier function, intestinal morphology, and cecal microflora. Based on initial BW, gender and litter, the pigs were given 5 treatments during a 14-d feeding experiment, including a basal diet (control), 3 diets with COS supplementation (200, 400, or 600 mg/kg), and a diet with colistin sulfate (CSE) supplementation (20 mg/kg). Six randomly selected pigs from each treatment were used to collect serum, duodenal, jejunal, ileal, and cecal samples on d 7 and 14 postweaning. From d 1 to 7 postweaning, pigs fed COS or CSE had greater ADG and ADFI compared with the control pigs. From d 1 to 14, diets with either 400 or 600 mg/kg COS, or 20 mg/kg CSE increased (P < 0.05) ADG and G:F compared with the control diet. No significant differences were observed in ADG, ADFI, and G:F between the pigs fed COS and CSE. Pigs fed either 400 or 600 mg/kg COS, or 20 mg/kg CSE had less (P < 0.05) diamine oxidase (DAO) in the serum, but greater concentration of (P < 0.05) DAO in jejunal mucosa, than the control pigs on d 7 postweaning. Treatments did not affect villous height and crypt depth of the duodenum, jejunum, or ileum. Pigs fed COS at 400 mg/kg had greater (P < 0.05) concentration of Bifidobacteria and Lactobacilli in the cecum than pigs fed the control diet and CSE diet on d 7 postweaning. Supplementation of COS or CSE decreased (P < 0.05) the population of cecal Staphylococcus aureus compared with the control diet on d 7 postweaning. The number of cecal Bifidobacteria in pigs fed 600 mg/kg COS was greater (P < 0.05) than that of pigs fed the control diet or CSE diet on d 14 postweaning. No significant differences were observed in Escherichia coli counts in the cecum among treatments. The present results indicate that dietary supplementation of COS at 400 or 600 mg/kg promotes growth performance and improves gut barrier function, increases the population of Bifidobacteria and Lactobacilli, and decreases S. aureus in the cecum of weanling pigs.
共有 180 头断奶仔猪（21 ± 3 日龄；5.98 ± 0.04 kg）用于研究壳寡糖（COS）对生长性能、肠道屏障功能、肠道形态和盲肠微生物区系的影响。基于初始 BW、性别和窝，在 14 天的饲养试验中，这些猪被分为 5 个处理组，包括基础日粮（对照组）、3 个添加 COS 的日粮（200、400 或 600 mg/kg）和 1 个添加硫酸粘菌素（CSE）的日粮（20 mg/kg）。在断奶后第 7 和 14 天，每个处理组中随机选择 6 头猪收集血清、十二指肠、空肠、回肠和盲肠样品。从断奶后第 1 天到第 7 天，添加 COS 或 CSE 的猪与对照组相比，ADG 和 ADFI 更高。从断奶后第 1 天到第 14 天，添加 400 或 600 mg/kg COS 或 20 mg/kg CSE 的日粮与对照组相比，ADG 和 G:F 更高（P < 0.05）。添加 COS 或 CSE 的猪的 ADG、ADFI 和 G:F 之间没有显著差异。与对照组相比，断奶后第 7 天，添加 400 或 600 mg/kg COS 或 20 mg/kg CSE 的猪血清中二胺氧化酶（DAO）含量较低（P < 0.05），但空肠黏膜中 DAO 浓度较高（P < 0.05）。在断奶后第 7 天，各处理组的十二指肠、空肠和回肠的绒毛高度和隐窝深度没有差异。添加 400 mg/kg COS 的猪盲肠中双歧杆菌和乳酸杆菌的浓度高于对照组和 CSE 组（P < 0.05）。与对照组相比，断奶后第 7 天，添加 COS 或 CSE 降低了（P < 0.05）盲肠金黄色葡萄球菌的数量。断奶后第 14 天，添加 600 mg/kg COS 的猪盲肠中双歧杆菌数量高于对照组和 CSE 组（P < 0.05）。各处理组盲肠中大肠杆菌数量没有差异。结果表明，日粮中添加 400 或 600 mg/kg COS 可促进生长性能，改善肠道屏障功能，增加双歧杆菌和乳酸杆菌的数量，降低断奶仔猪盲肠中金黄色葡萄球菌的数量。
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