Fountoulakis Stelios, Vartholomatos George, Kolaitis Nikolaos, Frillingos Stathis, Philippou George, Tsatsoulis Agathocles
Department of Endocrinology, Medical School, University of Ioannina, 45110 Ioannina, Greece.
Eur J Endocrinol. 2008 Jun;158(6):853-9. doi: 10.1530/EJE-08-0092.
To examine whether the Fas system apoptotic molecules are differentially expressed in Graves' disease (GD) and Hashimoto's thyroiditis (HT), the two opposite phenotypes of autoimmune thyroid disease (AITD).
The expression of Fas and Fas ligand (FasL) on peripheral CD4 and CD8 lymphocytes, and non-lymphoid immune cells as well as their soluble forms in serum from untreated patients with GD and HT were evaluated.
Flow cytometry was performed for the study of peripheral immune cells from 70 newly diagnosed patients with AITD (55 with HT and 15 with GD) and 20 controls. ELISA was used for the measurement of soluble Fas (sFas) in serum samples from a subgroup of 35 AITD patients.
An increase in the proportion of CD4 and CD8 cells expressing Fas was found in both GD and HT, albeit with some differences, when compared with controls. Importantly, in GD patients, the intensity of Fas expression on CD4 and CD8 lymphocytes was reduced and sFas levels in serum were simultaneously increased when compared with HT patients and controls.
The Fas system apoptotic molecules appear to be differentially expressed on peripheral lymphocytes in the two opposite phenotypes of AITD.
研究Fas系统凋亡分子在自身免疫性甲状腺疾病（AITD）的两种相反表型，即格雷夫斯病（GD）和桥本甲状腺炎（HT）中是否存在差异表达。
评估未经治疗的GD和HT患者外周血CD4和CD8淋巴细胞、非淋巴细胞免疫细胞上Fas和Fas配体（FasL）的表达情况以及血清中它们的可溶性形式。
对70例新诊断的AITD患者（55例HT患者和15例GD患者）及20例对照者的外周免疫细胞进行流式细胞术检测。采用酶联免疫吸附测定法（ELISA）检测3�例AITD患者亚组血清样本中的可溶性Fas（sFas）。
与对照组相比，GD和HT患者中表达Fas的CD4和CD8细胞比例均有所增加，尽管存在一些差异。重要 的是，与HT患者和对照组相比，GD患者CD4和CD8淋巴细胞上Fas的表达强度降低，血清中sFas水平同时升高。
Fas系统凋亡分子在AITD的两种相反表型的外周淋巴细胞上似乎存在差异表达。
