Ji Xuemeng, Lu Ping, Hu Yaozhong, Xue Juan, Wu Jing, Zhang Bowei, Zhang Yan, Dong Lu, Lv Huan, Wang Shuo
Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin, China.
Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical Collage, Tianjin, China.
Front Microbiol. 2021 Jun 25;12:687243. doi: 10.3389/fmicb.2021.687243. eCollection 2021.
Virulence traits and antibiotic resistance are frequently provided by genes located on plasmids. However, experimental verification of the functions of these genes is often lacking due to a lack of related experimental technology. In the present study, an integrated suicide vector was used to efficiently and specifically delete a bacterial endogenous plasmid in . The pESA3 plasmid was removed from BAA-894, and we confirmed that this plasmid contributes to the invasion and virulence of this strain. In addition, the pGW1 plasmid was expunged from GZcsf-1, and we confirmed that this plasmid confers multidrug resistance. We further screened plasmid-curing agents and found that -coumaric acid had a remarkable effect on the curing of pESA3 and pGW1 at sub-inhibitory concentrations. Our study investigated the contribution of endogenous plasmids pESA3 and pGW1 by constructing plasmid-cured strains using suicide vectors and suggested that -coumaric acid can be a safe and effective plasmid-curing agent for .
毒力特性和抗生素抗性通常由位于质粒上的基因提供。然而，由于缺乏相关实验技术，这些基因功能的实验验证往往不足。在本研究中，使用一种整合自杀载体高效且特异性地删除了细菌内源质粒。从BAA - 894中去除了pESA3质粒，并且我们证实该质粒有助于该菌株的侵袭和毒力。此外，从GZcsf - 1中消除了pGW1质粒，并且我们证实该质粒赋予多重耐药性。我们进一步筛选了质粒消除剂，发现对香豆酸在亚抑制浓度下对pESA3和pGW1的消除有显著效果。我们的研究通过使用自杀载体构建质粒消除菌株，研究了内源质粒pESA3和pGW1的作用，并表明对香豆酸可以是一种安全有效的质粒消除剂。
