骨髓间充质干细胞能够介导受损组织中的乙型肝炎病毒感染。
Bone marrow-derived mesenchymal stem cells are capable of mediating hepatitis B virus infection in injured tissues.
机构信息
Department of Cardiology, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
出版信息
J Viral Hepat. 2008 Aug;15(8):607-14. doi: 10.1111/j.1365-2893.2008.00978.x. Epub 2008 May 27.
UNLABELLED
We have previously showed that endothelial progenitor cells (EPCs) through uptake of hepatitis B virus (HBV) may play a critical role in mediating extrahepatic HBV diseases. However, it remains to be elucidated whether mesenchymal stem cells (MSCs) are capable of mediating HBV trans-infection into extrahepatic tissues.
METHODS AND RESULTS
In this study, we showed that HBV antigens, HBV DNA and the viral particles were detected in MSCs after 3 days virus challenge. Neither HBV covalently closed circular DNA nor pregenomic RNA were detected in MSCs. Intravenously transplantation of HBV-exposed MSCs into myocardial infarction mouse model resulted in incorporation of HBV into injured heart and other damaged tissues.
CONCLUSION
These results indicate that MSCs could serve as an additional extrahepatic virus reservoir, which may play a role at least in part in mediating HBV trans-infection into the injured tissues through the process of MSCs recruitment.
未注明
我们之前已经表明，内皮祖细胞（EPCs）通过摄取乙型肝炎病毒（HBV）可能在介导肝外 HBV 疾病中发挥关键作用。然而，间充质干细胞（MSCs）是否能够介导 HBV 转染到肝外组织仍有待阐明。
方法和结果
在这项研究中，我们表明，在病毒攻击 3 天后，HBV 抗原、HBV DNA 和病毒颗粒在 MSCs 中被检测到。MSCs 中既未检测到 HBV 共价闭合环状 DNA 也未检测到前基因组 RNA。将暴露于 HBV 的 MSCs 静脉内移植到心肌梗死小鼠模型中，导致 HBV 整合到受损心脏和其他受损组织中。
结论
这些结果表明，MSCs 可以作为肝外病毒储存库，至少在一定程度上可以通过 MSCs 募集的过程介导 HBV 转染到受损组织。
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