Department of Pediatrics, University of Utah, Salt Lake City, UT, USA.
Pediatr Rheumatol Online J. 2008 May 28;6:8. doi: 10.1186/1546-0096-6-8.
Cytokines play important roles in mediating inflammation in autoimmunity. Several cytokines are elevated in serum and synovial fluid samples from children with Juvenile Idiopathic Arthritis (JIA). Soluble CD154 (sCD154) is elevated in other autoimmune disorders, but has not been characterized in JIA. Our objectives were to determine if sCD154 is elevated in JIA, and to examine correlations between sCD154 and other inflammatory cytokines.
Serum from 77 children with JIA and 81 pediatric controls was analyzed for interleukin (IL)1beta, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL13, sCD154, interferon-gamma (IFNgamma), soluble IL2 receptor (sIL2R), and tumor necrosis factor-alpha (TNFalpha), using the Luminex Multi-Analyte Profiling system. Differences in levels of cytokines between cases and controls were analyzed. Logistic regression was also performed.
sCD154 was significantly elevated in cases compared to controls (p < 0.0001). IL1beta, IL5, IL6, IL8, IL13, IFNgamma, sIL2R, and TNFalpha were also significantly elevated in JIA. Levels of sCD154 were highly correlated with IL1beta, IL6, IL8, and TNFalpha (p < 0.0001). Logistic regression analysis suggested that IL6 (odds ratio (OR): 1.4, p < 0.0001), sCD154 (OR: 1.1, p < 0.0001), and TNFalpha (OR: 1.1, p < 0.005) were positively associated with JIA, while IL10 (OR: 0.5, p < 0.002) was protective. sCD154 was elevated in all JIA subtypes, with highest levels among more severe subtypes. IL1beta, IL6, IL8, sIL2R and TNFalpha were also elevated in several JIA subtypes.
Serum levels of sCD154, IL1beta, IL6, IL8, sIL2R and TNFalpha are elevated in most JIA subtypes, suggesting a major role for sCD154, and these cytokines and cytokine receptors in the pathogenesis of JIA.
细胞因子在介导自身免疫中的炎症中发挥重要作用。几种细胞因子在儿童幼年特发性关节炎（JIA）的血清和滑液样本中升高。可溶性 CD154（sCD154）在其他自身免疫性疾病中升高，但在 JIA 中尚未得到描述。我们的目的是确定 sCD154 是否在 JIA 中升高，并检查 sCD154 与其他炎症细胞因子之间的相关性。
使用 Luminex 多分析物分析系统分析 77 例 JIA 患儿和 81 例儿科对照者的血清中白细胞介素（IL）1β、IL2、IL4、IL5、IL6、IL8、IL10、IL12、IL13、sCD154、干扰素-γ（IFNγ）、可溶性 IL2 受体（sIL2R）和肿瘤坏死因子-α（TNFα）。分析病例与对照组之间细胞因子水平的差异。还进行了逻辑回归分析。
sCD154 在病例组中明显高于对照组（p < 0.0001）。IL1β、IL5、IL6、IL8、IL13、IFNγ、sIL2R 和 TNFα在 JIA 中也明显升高。sCD154 水平与 IL1β、IL6、IL8 和 TNFα高度相关（p < 0.0001）。逻辑回归分析表明，IL6（比值比（OR）：1.4，p < 0.0001）、sCD154（OR：1.1，p < 0.0001）和 TNFα（OR：1.1，p < 0.005）与 JIA 呈正相关，而 IL10（OR：0.5，p < 0.002）具有保护作用。sCD154 在所有 JIA 亚型中均升高，在更严重的亚型中升高最明显。IL1β、IL6、IL8、sIL2R 和 TNFα在几种 JIA 亚型中也升高。
sCD154、IL1β、IL6、IL8、sIL2R 和 TNFα 的血清水平在大多数 JIA 亚型中升高，表明 sCD154 以及这些细胞因子和细胞因子受体在 JIA 的发病机制中起重要作用。
