Zhang Jing, Chen Xi Guang, Peng Wei Bing, Liu Cheng Sheng
College of Marine Life Science, Ocean University of China, Qingdao, PR China.
Nanomedicine. 2008 Sep;4(3):208-14. doi: 10.1016/j.nano.2008.03.006. Epub 2008 May 27.
The aim of the present study was to evaluate cellular uptake of oleoyl-chitosan (OCH) nanoparticles by using A549 cells, a human lung carcinoma cell line, for drug and gene delivery applications. In this study, self-assembled OCH nanoparticles encapsulating a fluorescent marker molecule, fluorescein isothiocyanate (FITC), were prepared and characterized. The effects of particle size, concentration, and incubation time on the cellular uptake of the nanoparticles (FITC-OCH nanoparticles) were quantified by spectrofluorometric measurement and confirmed using fluorescence microscopy studies. The nanoparticles were taken up by the cells, and levels of binding and uptake increased with the decrease of particle size and the increase of particle concentration and incubation time. These results implied that the OCH nanoparticles have great potential to be applied as a drug carrier system to deliver drugs into the cells.
本研究的目的是评估油酰壳聚糖（OCH）纳米颗粒被人肺癌细胞系A549细胞摄取的情况，用于药物和基因递送应用。在本研究中，制备并表征了包裹荧光标记分子异硫氰酸荧光素（FITC）的自组装OCH纳米颗粒。通过荧光分光光度法测量定量了粒径、浓度和孵育时间对纳米颗粒（FITC-OCH纳米颗粒）细胞摄取的影响，并通过荧光显微镜研究进行了确认。细胞摄取了纳米颗粒，结合和摄取水平随着粒径的减小、颗粒浓度的增加以及孵育时间的延长而升高。这些结果表明，OCH纳米颗粒作为一种将药物递送至细胞内的药物载体系统具有巨大的应用潜力。
