Akusjarvi Goran
Department of Medical Biochemistry and Microbiology, BMC, Uppsala University, 751 23 Uppsala, Sweden.
Front Biosci. 2008 May 1;13:5006-15. doi: 10.2741/3059.
Adenovirus makes extensive use of alternative RNA splicing to produce a complex set of spliced mRNAs during replication. The accumulation of viral mRNAs is subjected to a temporal regulation, a mechanism that ensures that proteins that are needed at certain stages of the virus life cycle are produced in a timely fashion. The complex interactions between the virus and the host cell RNA splicing machinery has been studied in detail during the last decade. These studies have resulted in the characterization of two viral proteins, E4-ORF4 and L4-33K, that adenovirus uses to remodel the host cell RNA splicing machinery. Here I will review the current knowledge of how mRNA expression from the adenovirus major late transcription unit is controlled with a particular emphasis on how cis-acting sequence element, trans-acting factors and mechanisms regulating adenovirus major late L1 alternative RNA splicing is controlled.
腺病毒在复制过程中广泛利用可变RNA剪接来产生一组复杂的剪接mRNA。病毒mRNA的积累受到时间调控，这是一种确保在病毒生命周期特定阶段所需的蛋白质能够及时产生的机制。在过去十年中，对病毒与宿主细胞RNA剪接机制之间的复杂相互作用进行了详细研究。这些研究已鉴定出两种病毒蛋白，即E4-ORF4和L4-33K，腺病毒利用它们重塑宿主细胞RNA剪接机制。在此，我将综述目前关于腺病毒主要晚期转录单位mRNA表达如何受到控制的知识，特别强调顺式作用序列元件、反式作用因子以及调控腺病毒主要晚期L1可变RNA剪接的机制是如何受到控制的。
