Cole K A, Krizman D B, Emmert-Buck M R
Pathogenetics Unit, Laboratory of Pathology, National Cancer Institute, Bethesda, Maryland 20892, USA.
Nat Genet. 1999 Jan;21(1 Suppl):38-41. doi: 10.1038/4466.
Gene expression microarrays hold great promise for studies of human disease states. There are significant technical issues specific to utilizing clinical tissue samples which have yet to be rigorously addressed and completely overcome. Precise, quantitative measurement of gene expression profiles from specific cell populations is at hand, offering the scientific community the first comprehensive view of the in vivo molecular anatomy of normal cells and their diseased counterparts. Here, we propose a model for integrating-in three dimensions-expression data obtained using the microarray.
基因表达微阵列在人类疾病状态研究方面具有巨大潜力。在利用临床组织样本时存在一些特定的重大技术问题，这些问题尚未得到严格解决和彻底克服。对特定细胞群体的基因表达谱进行精确、定量测量即将实现，这为科学界提供了对正常细胞及其病变对应细胞的体内分子解剖结构的首个全面视角。在此，我们提出一个用于在三维空间中整合使用微阵列获得的表达数据的模型。
