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泡沫病毒逆转录酶的脱氧核苷三磷酸掺入机制：对泡沫病毒细胞嗜性的影响

Deoxynucleoside triphosphate incorporation mechanism of foamy virus (FV) reverse transcriptase: implications for cell tropism of FV.

作者信息

Santos-Velazquez Jose, Kim Baek

机构信息

Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, NY 14642, USA.

出版信息

J Virol. 2008 Aug;82(16):8235-8. doi: 10.1128/JVI.00088-08. Epub 2008 May 28.

DOI:10.1128/JVI.00088-08
PMID:18508890
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2519572/
Abstract

Here, we investigated the pre-steady-state deoxynucleoside triphosphate (dNTP) incorporation kinetics of primate foamy virus (PFV) reverse transcriptase (RT) in comparison with those of HIV-1 and MuLV RTs. PFV RT displayed a drastic reduction in primer extension at low dNTP concentrations where HIV-1 RT remains highly active, indicating a low dNTP binding affinity in the case of PFV RT. Indeed, kinetic analysis showed that, as observed with MuLV RT, PFV RT exhibits approximately 10 to 80 times lower dNTP binding affinity than HIV-1 RT. These three RTs, however, show similar catalytic activities. In conclusion, PFV RT displays mechanistic distinctions in comparison to HIV-1 RT and shares close similarity to MuLV RT.

摘要

在此，我们研究了灵长类泡沫病毒（PFV）逆转录酶（RT）与HIV-1和莫洛尼鼠白血病病毒（MuLV）RT相比的前稳态脱氧核苷三磷酸（dNTP）掺入动力学。在低dNTP浓度下，PFV RT的引物延伸急剧减少，而HIV-1 RT在此浓度下仍保持高活性，这表明PFV RT的dNTP结合亲和力较低。实际上，动力学分析表明，正如在MuLV RT中观察到的那样，PFV RT的dNTP结合亲和力比HIV-1 RT低约10至80倍。然而，这三种RT显示出相似的催化活性。总之，与HIV-1 RT相比，PFV RT表现出机制上的差异，并且与MuLV RT具有密切的相似性。