Osman Nussieba A, A/Rahman Mahasin E, Ali A S, Fadol M A
Department of Pathology, Parasitology and Microbiology, College of Veterinary Medicine & Animal Production, Sudan University of Science & Technology, P.O.Pox 204, Khartoum North, Sudan.
Trop Anim Health Prod. 2008 Jun;40(5):363-8. doi: 10.1007/s11250-007-9106-1.
AGPT and HA tests were employed for rapid diagnosis of PPRV infection in sheep and goats in Sudan. Forty lymph nodes and spleen samples from suspected cases of PPR in both sheep and goats were examined by AGPT and HA tests for detection of PPRV antigen. Viral antigen was detected from (77.5%) of the samples tested by AGPT and (92.5%) tested by HA test. The results of both tests revealed that HA test was more sensitive than AGPT for detection of PPRV antigen (Kappa statistics 0.4366). Another advantage of the HA test over AGPT was that it can differentiate PPRV from RPV. Thus the HA test represents a quick, easy, simple, cheap and reliable confirmatory test for the diagnosis of PPR and differential diagnosis of PPRV and RPV. The HA test was carried out using chicken, goat and pig RBCs. Chicken RBCs were found to be the most sensitive for detection of PPRV antigen, followed by goat then pig RBCs. The HA time when using chicken RBCs was 20-25 minutes, using goat RBCs was 25-30 minutes and using pig RBCs was 40-45 minutes. The distribution of PPR infection in four different regions of Sudan was investigated.
AGPT和HA检测用于苏丹绵羊和山羊中PPRV感染的快速诊断。对来自绵羊和山羊PPR疑似病例的40个淋巴结和脾脏样本进行AGPT和HA检测，以检测PPRV抗原。通过AGPT检测的样本中有77.5%检测到病毒抗原，通过HA检测的样本中有92.5%检测到病毒抗原。两种检测结果均显示，在检测PPRV抗原方面，HA检测比AGPT更敏感（卡方统计值为0.4366）。HA检测相对于AGPT的另一个优势在于它能够区分PPRV和RPV。因此，HA检测是一种用于PPR诊断以及PPRV和RPV鉴别诊断的快速、简便、廉价且可靠的确诊检测方法。HA检测使用鸡、山羊和猪的红细胞进行。结果发现，鸡红细胞对PPRV抗原检测最为敏感，其次是山羊红细胞，然后是猪红细胞。使用鸡红细胞时HA检测时间为20 - 25分钟，使用山羊红细胞时为25 - 30分钟，使用猪红细胞时为40 - 45分钟。对苏丹四个不同地区的PPR感染分布情况进行了调查。
