Osman Nussieba A, Ali A S, A/Rahman Mahasin E, Fadol M A
Department of Pathology, Parasitology and Microbiology, College of Veterinary Medicine & Animal Production, Sudan University of Science & Technology, P.O.Pox 204, Khartoum North, Sudan.
Trop Anim Health Prod. 2009 Oct;41(7):1449-53. doi: 10.1007/s11250-009-9333-8.
Counter immnuo-electrophoresis (CIEP) and Competitive ELISA (C-ELISA) tests were employed for seroprevalence of Peste des Petits Ruminants (PPR) infection in Sudan. The result of both tests showed high prevalence of PPRV antibodies in sheep and goats sera collected from six different regions of Sudan. Of the 519 serum samples examined for the presence of PPRV antibodies 307(59.15%) were positive by CIEP while 263(50.67%) were positive by C-ELISA. CIEP technique was shown to be more sensitive than C-ELISA technique for detection of PPRV antibodies (Kappa statistics 0.259). C-ELISA allowed rapid, simple, specific, sensitive and differential sero-diagnosis of PPRV and RPV in sheep, goats and cattle. CIEP is, unlike competitive ELISA, is group-specific test and can not differentiate between PPR and RP infections. Despite its low specificity CIEP can be a useful indicative screening test for PPRV antibodies in flocks that neither been vaccinated nor otherwise exposed to PPR or RP virus. Results obtained suggest that CIEP, like the HI test, could be a useful screening test where it is not possible to use C-ELISA.
采用对流免疫电泳（CIEP）和竞争ELISA（C-ELISA）检测苏丹小反刍兽疫（PPR）感染的血清流行率。两项检测结果均显示，从苏丹六个不同地区采集的绵羊和山羊血清中，PPRV抗体的流行率很高。在检测PPRV抗体的519份血清样本中，307份（59.15%）通过CIEP检测呈阳性，而263份（50.67%）通过C-ELISA检测呈阳性。结果表明，CIEP技术在检测PPRV抗体方面比C-ELISA技术更敏感（卡方统计值为0.259）。C-ELISA可对绵羊、山羊和牛群中的PPRV和RPV进行快速、简单、特异、灵敏的鉴别血清诊断。与竞争ELISA不同，CIEP是一种群特异性检测方法，无法区分PPR和RP感染。尽管CIEP特异性较低，但对于既未接种疫苗也未接触过PPR或RP病毒的羊群，它可作为一种有用的PPRV抗体指示性筛查检测方法。所得结果表明，与血凝抑制试验（HI）一样，在无法使用C-ELISA的情况下，CIEP可能是一种有用的筛查检测方法。
