Marcante M L, Venuti A
Laboratory of Virology, Regina Elena Institute for Cancer Research, Rome, Italy.
Eur J Cancer. 1991;27(2):187-90. doi: 10.1016/0277-5379(91)90484-u.
Paraffin-embedded tumour sections were used for the polymerase chain reaction (PCR) with three primer sets that amplify specific regions of human papillomavirus (HPV) types 11, 16 and 18. The positive samples were confirmed by hybridisation of the amplified sequences with the specific HPV probes. In all screened metastases the same viral sequences were found as in the primary tumour. HPV 16 was the most frequently detected virus in genital tract tumours. In a metastatic ovary carcinoma with unknown primary site HPV 16 DNA was observed. Moreover, pelvic lymph nodes with no microscopic evidence of metastases contained HPV DNA of the same subtype as the primary tumour. Thus, the HPV DNA detected by PCR is a useful indicator of neoplastic cells in the earlier stages of invasiveness. The finding of specific HPVs in the metastatic lesions could also provide information about the location of the primary neoplasia.
石蜡包埋的肿瘤切片用于聚合酶链反应（PCR），使用了三组引物来扩增人乳头瘤病毒（HPV）11、16和18型的特定区域。通过将扩增序列与特异性HPV探针杂交来确认阳性样本。在所有筛查的转移灶中，发现的病毒序列与原发肿瘤中的相同。HPV 16是生殖道肿瘤中最常检测到的病毒。在一例原发部位不明的转移性卵巢癌中观察到了HPV 16 DNA。此外，没有显微镜下转移证据的盆腔淋巴结含有与原发肿瘤相同亚型的HPV DNA。因此，通过PCR检测到的HPV DNA是侵袭早期肿瘤细胞的有用指标。在转移灶中发现特定的HPV也可为原发性肿瘤的位置提供信息。
