Yuan Xiaofei, Iijima Michihiro, Oishi Motoi, Nagasaki Yukio
Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Ten-noudai, Tsukuba, Ibaraki 305-8753, Japan.
Langmuir. 2008 Jun 1;24(13):6903-9. doi: 10.1021/la7039288. Epub 2008 May 30.
Enzyme/polymer/gold nanoparticle hybrids, called "nanozymes", were prepared and structurally analyzed by dynamic light scattering (DLS), ultraviolet-visible spectroscopy, and zeta-potential and transmission electron microscopy (TEM) measurements, which showed that the nanozyme particles were mainly composed of a single gold nanoparticle, on whose surface the enzyme and polymer were coimmobilized. This kind of structure resulted in the high dispersion stability of the nanozyme under various conditions, accompanied by improved thermal stability of the enzyme.
被称为“纳米酶”的酶/聚合物/金纳米颗粒杂化物通过动态光散射（DLS）、紫外可见光谱、zeta电位和透射电子显微镜（TEM）测量进行了制备和结构分析，结果表明纳米酶颗粒主要由单个金纳米颗粒组成，酶和聚合物共固定在其表面。这种结构导致纳米酶在各种条件下具有高分散稳定性，同时酶的热稳定性也得到提高。
