Amon Sabine, Zamfir Alina D, Rizzi Andreas
Institute of Analytical Chemistry and Food Chemistry, University of Vienna, Vienna, Austria.
Electrophoresis. 2008 Jun;29(12):2485-507. doi: 10.1002/elps.200800105.
This review highlights recent developments in glycosylation analysis by modern MS in combination with CE based preseparation. Focused on CE-MS strategies aimed for glycotyping, the review addresses the detailed glycoform analysis of glycoproteins, glycopeptides, and proteoglycans. Glycoform analysis in the context of modern glycoproteomics is briefly addressed, as well. CZE, CIEF, and frontal analysis CE approaches hyphenated to high-resolution multistage MS for the detailed analysis of protein-linked glycan structures are overviewed in a comprehensive way. Advantages and limitations of various methodological approaches and techniques as well as mass spectrometric instrumentation are discussed in the particular context of glycoanalysis.
本综述重点介绍了现代质谱结合基于毛细管电泳的预分离技术在糖基化分析方面的最新进展。该综述聚焦于旨在进行糖型分析的毛细管电泳-质谱策略，探讨了糖蛋白、糖肽和蛋白聚糖的详细糖型分析。同时也简要介绍了现代糖蛋白质组学背景下的糖型分析。全面概述了与高分辨率多级质谱联用的毛细管区带电泳（CZE）、毛细管等聚焦电泳（CIEF）和前沿分析毛细管电泳方法，用于详细分析蛋白质连接的聚糖结构。在糖基分析的特定背景下，讨论了各种方法和技术以及质谱仪器的优缺点。
