Naoghare Pravin K, Kim Min Jung, Song Joon Myong
Research Institute of Pharmaceutical Sciences and College of Pharmacy, Seoul National University, Seoul 151-742, South Korea.
Anal Chem. 2008 Jul 15;80(14):5407-17. doi: 10.1021/ac800452x. Epub 2008 May 31.
Implementation of quantitative analytical approaches to image-based cellular assays remains a major challenge. We disclose a tool to achieve automatic rapid quantitative cellular imaging analysis based on uniform threshold intensity distribution. An acousto-optic tunable filter-based, quantitative multivariate imaging cytometer was set up to elucidate drug-induced cell death dynamics via cell viability and apoptosis/necrosis measurements in the human myeloid leukemia cell line, HL-60. Cells were treated with various drugs (camptothecin, naringenin, sodium salicylate) at different concentrations and time intervals. The developed protocol can directly depict and quantitate targeted cellular moieties, subsequently enabling a method that is applicable to various cellular assays with special reference to next generation drug discovery screening. This may also complement certain flow-cytometric measurements in studying quantitative physiology of cellular systems.
将定量分析方法应用于基于图像的细胞分析仍然是一项重大挑战。我们公开了一种基于均匀阈值强度分布实现自动快速定量细胞成像分析的工具。建立了一种基于声光可调谐滤波器的定量多变量成像细胞仪，通过对人髓系白血病细胞系HL-60的细胞活力和凋亡/坏死测量来阐明药物诱导的细胞死亡动力学。细胞在不同浓度和时间间隔下用各种药物（喜树碱、柚皮素、水杨酸钠）处理。所开发的方案可以直接描绘和定量靶向细胞部分，随后形成一种适用于各种细胞分析的方法，特别适用于下一代药物发现筛选。这也可能在研究细胞系统的定量生理学方面补充某些流式细胞术测量。
