Senthilselvan Ambikaipakan, Rennie Donna, Chénard Liliane, Burch Lauranell H, Babiuk Lorne, Schwartz David A, Dosman James A
Department of Public Health Sciences, School of Public Health, University of Alberta, Edmonton, Alberta, Canada.
Ann Allergy Asthma Immunol. 2008 May;100(5):463-8. doi: 10.1016/S1081-1206(10)60472-3.
The response to innate immune stimuli seems to be critical to conditioning adaptive immunity. Early exposure to endotoxin initiates immune responses that have been shown to alter the risk of asthma and allergic diseases. The toll-like receptor 4 (TLR4) gene encodes the principal innate immunity receptor in humans for bacterial endotoxin. Polymorphisms in the TLR4 gene may regulate the effects of endotoxin exposure and could play a role in the development of asthma and atopy-related phenotypes.
To investigate the association between TLR4 polymorphisms and allergic phenotypes in nonsmokers.
The data from 915 nonsmoking students were available for the study. The TLR4 299 and 399 polymorphisms were genotyped using mouthwash samples. The TLR4 299 and 399 polymorphisms were grouped together to define the TLR4 polymorphic group. Skin prick tests were conducted in a subgroup of healthy participants. A brief questionnaire was administered to determine demographic characteristics and chronic health conditions.
The prevalence of hay fever was 0% in the TLR4 polymorphic group and 7.5% in the wild-type group (P = .01). After controlling for age group and sex using logistic regression, the odds of having hay fever were reduced by 88% (P = .009) in the TLR4 polymorphic group compared with the wild-type group. In a subgroup analysis, the association between TLR4 polymorphisms and atopy was only observed among females.
To our knowledge, this study is the first to report an association between TLR4 polymorphisms and atopy-related phenotypes in a nonsmoking population. Further investigation of the role of TLR4 polymorphisms in asthma and atopy-related phenotypes is warranted.
对先天免疫刺激的反应似乎对调节适应性免疫至关重要。早期接触内毒素会引发免疫反应，这些反应已被证明会改变哮喘和过敏性疾病的风险。Toll样受体4（TLR4）基因编码人类中细菌内毒素的主要先天免疫受体。TLR4基因的多态性可能调节内毒素暴露的影响，并可能在哮喘和特应性相关表型的发展中起作用。
研究TLR4多态性与非吸烟者过敏性表型之间的关联。
915名非吸烟学生的数据可用于该研究。使用漱口水样本对TLR4 299和399多态性进行基因分型。将TLR4 299和399多态性组合在一起以定义TLR4多态性组。在一组健康参与者中进行皮肤点刺试验。发放一份简短问卷以确定人口统计学特征和慢性健康状况。
TLR4多态性组中花粉症的患病率为0%，野生型组中为7.5%（P = 0.01）。使用逻辑回归控制年龄组和性别后，与野生型组相比，TLR4多态性组患花粉症的几率降低了88%（P = 0.009）。在亚组分析中，仅在女性中观察到TLR4多态性与特应性之间的关联。
据我们所知，本研究首次报道了非吸烟人群中TLR4多态性与特应性相关表型之间的关联。有必要进一步研究TLR4多态性在哮喘和特应性相关表型中的作用。
