Sikri K L, Hoyes A D, Barber P, Jagessar H
J Anat. 1981 Oct;133(Pt 3):425-42.
Immunofluorescence and immunoperoxidase techniques were used to study the distribution of substance P-like immunoreactivity in the intramural nerve plexuses of the guinea-pig ureter. In light microscopical preparations, immunoreactivity was observed in plexuses related to the muscle coat as well as in plexuses in the submucosa and beneath the epithelium. Ultrastructural examination showed that the immunoreactivity was located primarily in axons in the nerves. In perfusion-fixed specimens, there was evidence of its presence both in axons with terminals containing mainly large dense-cored vesicles and in axons with terminals containing mainly small vesicles. The presence of substance P-like immunoreactivity in axons with terminals containing mainly large dense-cored vesicles was supported by examination of specimens treated in vitro with capsaicin. In these specimens, the axons were dilated and showed a number of other changes in fine structure. There was also a substantial reduction in the amount of immunoreactivity in the nerve plexuses and the dilated axons contained little if any reaction product. The possibility that axons which contained large amounts of reaction product after treatment with capsaicin represented axons with terminals containing mainly small vesicles was discussed. Comparison of the distribution of the different types of small vesicle-containing terminal identified in glutaraldehyde-fixed material with that of axons containing reaction product suggested that the immunoreactivity present in such axons was located in those in which the small vesicles in the terminals were clear rather than those in which the vesicles contained dense material.
采用免疫荧光和免疫过氧化物酶技术研究了豚鼠输尿管壁内神经丛中P物质样免疫反应性的分布。在光学显微镜标本中，在与肌层相关的神经丛以及黏膜下层和上皮下的神经丛中均观察到免疫反应性。超微结构检查显示，免疫反应性主要位于神经中的轴突内。在灌注固定标本中，有证据表明其存在于主要含有大的致密核心囊泡的终末轴突以及主要含有小囊泡的终末轴突中。用辣椒素体外处理标本的检查结果支持了主要含有大的致密核心囊泡的终末轴突中存在P物质样免疫反应性这一观点。在这些标本中，轴突扩张并在精细结构上显示出许多其他变化。神经丛中的免疫反应性数量也大幅减少，扩张的轴突中几乎没有反应产物。讨论了用辣椒素处理后含有大量反应产物的轴突是否代表主要含有小囊泡的终末轴突的可能性。将在戊二醛固定材料中鉴定出的不同类型的含小囊泡终末的分布与含有反应产物的轴突的分布进行比较，结果表明，此类轴突中的免疫反应性位于终末小囊泡清晰的轴突中，而非小囊泡含有致密物质的轴突中。
