Yiğit Demet, Yiğit Nimet, Ozgen Ufuk
Department of Science Education, Erzincan Education Faculty, University of Erzincan, Erzincan, Turkey.
Mycoses. 2009 Mar;52(2):135-40. doi: 10.1111/j.1439-0507.2008.01552.x. Epub 2008 Jun 3.
Methanol and chloroform extracts obtained from eight plant species belonging to six families, which were selected depending on their use in Turkish folk medicine, including Mentha longifolia L. (Labiatae), Mentha piperita L. Hudson (Labiatae), Prongos ferulaceae (Umbelliferae), Galium verum L. (Rubiaceae), Salvia limbata C. A Meyer (Labiatae), Artemisia austriaca Jacq. (Artemiceae), Plantago lanceolata L. (Plantaginaceae) and Urtica dioica L. (Urticaceae) were evaluated for their in vitro anticandidal activity. The anticandidal activity of extracts against 99 human pathogenic clinical isolates belonging to 35 Candida albicans, 33 Candida tropicalis and 31 Candida glabrata and standard strains of Candida spp. (C. albicans ATCC 10231, C. glabrata ATCC 80030 and C. tropicalis ATCC 22019) were tested by disc diffusion method and the active extracts were assayed for the minimal inhibitory concentration (MIC). Chloroform extracts of plants have no inhibitory effect against both clinical and standard strains of Candida spp., whereas methanol extracts exhibited good activity. Among the plants tested, M. piperita showed the highest anticandidal activity with 12.3 mm inhibition zone and 1.25 mg ml(-1) MIC value against C. albicans, M. longifolia, P. lanceolata and A. austriaca also displayed activity against C. albicans and C. tropicalis.
从六个科的八种植物中获得的甲醇和氯仿提取物，这些植物是根据它们在土耳其民间医学中的用途挑选出来的，包括长叶薄荷（唇形科）、胡椒薄荷（唇形科）、阿魏（伞形科）、蓬子菜（茜草科）、边缘鼠尾草（唇形科）、奥地利艾（菊科）、披针叶车前（车前科）和异株荨麻（荨麻科），对其体外抗念珠菌活性进行了评估。通过纸片扩散法测试了提取物对属于35株白色念珠菌、33株热带念珠菌和31株光滑念珠菌的99株人类致病临床分离株以及念珠菌属标准菌株（白色念珠菌ATCC 10231、光滑念珠菌ATCC 80030和热带念珠菌ATCC 22019）的抗念珠菌活性，并对活性提取物进行了最低抑菌浓度（MIC）测定。植物的氯仿提取物对念珠菌属的临床和标准菌株均无抑制作用，而甲醇提取物表现出良好的活性。在所测试的植物中，胡椒薄荷对白色念珠菌显示出最高的抗念珠菌活性，抑菌圈为12.3毫米，MIC值为1.25毫克/毫升，长叶薄荷、披针叶车前和奥地利艾对白色念珠菌和热带念珠菌也有活性。
