Hatai Hitoshi, Ochiai Kenji, Murakami Mariko, Imanishi Syunsuke, Tomioka Yukiko, Toyoda Takeshi, Ohashi Kazuhiko, Umemura Takashi
Laboratory of Comparative Pathology, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
J Vet Med Sci. 2008 May;70(5):469-74. doi: 10.1292/jvms.70.469.
Fowl glioma-inducing virus (FGV), which belongs to subgroup A of avian leukosis virus (ALV), is tumorigenic in the nervous system. In a zoological garden in Japan, approximately 40% of chickens, including Japanese fowls, were infected with FGV. Because this zoological garden plays a role as a major supplier of Japanese fowl for other zoological gardens, FGV infection is suspected to have spread among ornamental chickens. In this study, the prevalence of the disease was examined in a total of 129 chickens in three other zoological gardens by nested polymerase chain reaction (PCR), reverse transcription nested PCR and enzyme-linked immunosorbent assay. Twenty-six to 56 percent of the fowls in each of the examined gardens were positive by nested PCR. The phylogenetic analysis revealed that the 3' untranslated region, including the specific sequence of FGV, of the 14 isolated ALVs showed high sequence identity and a close relationship with FGV. In addition, the env gene of the isolates frequently showed mutations and deletions of nucleotides. These results suggest that FGV is prevalent among ornamental chickens kept in zoological gardens in Japan.
禽胶质瘤诱导病毒（FGV）属于禽白血病病毒（ALV）A亚群，在神经系统中具有致瘤性。在日本的一个动物园里，包括日本鸡在内的约40%的鸡感染了FGV。由于这个动物园是其他动物园日本鸡的主要供应源，怀疑FGV感染已在观赏鸡中传播。在本研究中，通过巢式聚合酶链反应（PCR）、逆转录巢式PCR和酶联免疫吸附测定，对另外三个动物园的总共129只鸡进行了该病的患病率检测。每个受检动物园中26%至56%的家禽通过巢式PCR呈阳性。系统发育分析显示，14株分离的ALV的3'非翻译区，包括FGV的特定序列，显示出高度的序列同一性，且与FGV关系密切。此外，分离株的env基因经常出现核苷酸突变和缺失。这些结果表明，FGV在日本动物园饲养的观赏鸡中普遍存在。
