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利用3T磁共振成像对大鼠脑出血模型神经干细胞的示踪研究

Tracking of neural stem cells in rats with intracerebral hemorrhage by the use of 3T MRI.

作者信息

Chang Nam Kyu, Jeong Yong Yeon, Park Jong Seong, Jeong Han Seong, Jang Sujeong, Jang Myung Joo, Lee Jae Hyuk, Shin Sang Soo, Yoon Woong, Chung Tae Woong, Kang Heoung Keun

机构信息

Department of Radiology, Chonnam National University Medical School, Gwang-ju, Korea.

出版信息

Korean J Radiol. 2008 May-Jun;9(3):196-204. doi: 10.3348/kjr.2008.9.3.196.

DOI:10.3348/kjr.2008.9.3.196
PMID:18525221
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2627254/
Abstract

OBJECTIVE

To access the feasibility of clinically available 3T MRI to detect the migration of labeled neural stem cells (NSCs) in intracerebral hemorrhage (ICH) in a rat model.

MATERIALS AND METHODS

The ethics committee of our institution approved this study. ICH was induced by the injection of collagenase type IV into the right striatum of ten Sprague-Dawley rats. Human NSCs conjugated with Feridex (super-paramagnetic iron oxide: SPIO) were transplanted into the left striatum one week after ICH induction. MRI was performed on a 3T scanner during the first, second, third, fourth, and sixth weeks post-transplantation. MRI was obtained using coronal T2- and T2*-weighted sequences. Two rats were sacrificed every week after in vivo MRI in order to analyze the histological findings.

RESULTS

ICH in the right striatum was detected by MRI one and two weeks after transplantation without migration of the NSCs. There was no migration of the NSCs as seen on the histological findings one week after transplantation. The histological findings two weeks after transplantation showed a small number of NSCs along the corpus callosum. On MRI three weeks after transplantation, there was a hypointense line along the corpus callosum and decreased signal intensity in the right periventricular region. Histological findings three weeks after transplantation confirmed the presence of the hypointense line representing SPIO-labeled NSCs. MRI four and six weeks after transplantation showed a hypointense spot in the right periventricular region. The histological findings four and six weeks after transplantation showed the presence of prominent NSCs in the right periventricular region.

CONCLUSION

3T MRI can detect the migration of NSCs in rats with ICH along the corpus callosum. Therefore, 3T MRI could be feasible for detecting the migration of NSCs in the clinical setting of stem cell therapy.

摘要

目的

探讨临床可用的3T磁共振成像（MRI）检测大鼠脑出血（ICH）模型中标记神经干细胞（NSCs）迁移的可行性。

材料与方法

本机构伦理委员会批准了本研究。通过向10只Sprague-Dawley大鼠的右侧纹状体注射IV型胶原酶诱导脑出血。在脑出血诱导1周后，将与菲立磁（超顺磁性氧化铁：SPIO）结合的人神经干细胞移植到左侧纹状体。在移植后的第1、2、3、4和6周，使用3T扫描仪进行MRI检查。采用冠状位T2加权和T2*加权序列获取MRI图像。在活体MRI检查后，每周处死2只大鼠以分析组织学结果。

结果

移植后1周和2周，MRI检测到右侧纹状体有脑出血，未见神经干细胞迁移。移植后1周的组织学检查结果未见神经干细胞迁移。移植后2周的组织学检查结果显示胼胝体周围有少量神经干细胞。移植后3周的MRI检查显示胼胝体有一条低信号线，右侧脑室周围区域信号强度降低。移植后3周的组织学检查结果证实存在代表SPIO标记神经干细胞的低信号线。移植后4周和6周的MRI检查显示右侧脑室周围区域有一个低信号点。移植后4周和6周的组织学检查结果显示右侧脑室周围区域有大量神经干细胞。

结论

3T MRI可检测ICH大鼠中神经干细胞沿胼胝体的迁移。因此，3T MRI在干细胞治疗的临床环境中检测神经干细胞的迁移可能是可行的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/290f/2627254/33254cea9695/kjr-9-196-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/290f/2627254/501c2a730f1a/kjr-9-196-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/290f/2627254/96f1781c9648/kjr-9-196-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/290f/2627254/a34c46e64dc6/kjr-9-196-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/290f/2627254/2745352cf399/kjr-9-196-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/290f/2627254/06a7cfbbb2b1/kjr-9-196-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/290f/2627254/b4f1a4de2e8e/kjr-9-196-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/290f/2627254/33254cea9695/kjr-9-196-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/290f/2627254/501c2a730f1a/kjr-9-196-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/290f/2627254/96f1781c9648/kjr-9-196-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/290f/2627254/a34c46e64dc6/kjr-9-196-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/290f/2627254/2745352cf399/kjr-9-196-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/290f/2627254/06a7cfbbb2b1/kjr-9-196-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/290f/2627254/b4f1a4de2e8e/kjr-9-196-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/290f/2627254/33254cea9695/kjr-9-196-g007.jpg

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本文引用的文献

1
Gene therapy: can neural stem cells deliver?基因治疗：神经干细胞能实现吗？
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2
In vivo and ex vivo MRI detection of localized and disseminated neural stem cell grafts in the mouse brain.小鼠脑内局部及播散性神经干细胞移植的体内和体外磁共振成像检测
Neuroimage. 2005 Jul 1;26(3):744-54. doi: 10.1016/j.neuroimage.2005.02.029. Epub 2005 Apr 7.
3
Autologous mesenchymal stem cell transplantation in stroke patients.中风患者的自体间充质干细胞移植
脑出血的骨髓源性细胞疗法的临床前和临床研究综述
Stem Cells Int. 2016;2016:4617983. doi: 10.1155/2016/4617983. Epub 2016 Sep 6.
4
Umbilical cord mesenchymal stem cells labeled with multimodal iron oxide nanoparticles with fluorescent and magnetic properties: application for in vivo cell tracking.具有荧光和磁性的多模态氧化铁纳米颗粒标记的脐带间充质干细胞：在体内细胞追踪中的应用
Int J Nanomedicine. 2014 Jan 8;9:337-50. doi: 10.2147/IJN.S53299. eCollection 2014.
5
Superparamagnetic iron oxide is suitable to label tendon stem cells and track them in vivo with MR imaging.超顺磁性氧化铁适合标记肌腱干细胞，并可通过磁共振成像在体内对其进行追踪。
Ann Biomed Eng. 2013 Oct;41(10):2109-19. doi: 10.1007/s10439-013-0802-x. Epub 2013 Apr 3.
6
Long-term magnetic resonance imaging of stem cells in neonatal ischemic injury.新生儿缺血性损伤中干细胞的长期磁共振成像。
Ann Neurol. 2011 Feb;69(2):282-91. doi: 10.1002/ana.22168. Epub 2010 Nov 22.
7
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Ann Neurol. 2005 Jun;57(6):874-82. doi: 10.1002/ana.20501.
4
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5
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6
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7
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9
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Stroke. 2003 Sep;34(9):2258-63. doi: 10.1161/01.STR.0000083698.20199.1F. Epub 2003 Jul 24.
10
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Radiology. 2003 Aug;228(2):480-7. doi: 10.1148/radiol.2281020638. Epub 2003 Jun 20.