Heston W E, Smith B, Parks W P
J Exp Med. 1976 Oct 1;144(4):1022-30. doi: 10.1084/jem.144.4.1022.
F1 and F2 and first backcross hybrids and second backcross families of the high mammary tumor incidence strain GR and the low incidence strain C57BL were examined for the segregation of mouse mammary tumor viral (MMTV) expression. Although GR has been reported to transmit MMTV as a single dominant gene, several lines of evidence suggest there are multiple genetic factors that influence MMTV expression, MMTV expression as measured by double antibody radioimmunoassay for MMTV p14 segregated in 106 first backcross progeny at a 60:40 ratio, intermediate between what would be expected for either a single or two gene hypothesis. In female second backcross progeny of either male or female first backcross, a heterogeneous pattern of expression was noted that does not fit any simple Mendalian pattern. From an analysis of serial lactations of first backcross and second backcross families, it appears that all hybrid females contain MMTV proviral information that may be expressed either at late lactations or in a variable proportion of progeny mice. These combined results are most consistent with a vertically transmitted genome regulated by multiple factors in these crosses.
对高乳腺肿瘤发病率品系GR和低发病率品系C57BL的F1、F2、第一代回交杂种及第二代回交家系进行了小鼠乳腺肿瘤病毒（MMTV）表达分离情况的检测。尽管已有报道称GR可作为单个显性基因传递MMTV，但多项证据表明存在多种影响MMTV表达的遗传因素。通过针对MMTV p14的双抗体放射免疫测定法检测到的MMTV表达，在106个第一代回交后代中以60:40的比例分离，这一比例介于单基因或双基因假说预期的比例之间。在雄性或雌性第一代回交的雌性第二代回交后代中，观察到一种异质性的表达模式，不符合任何简单的孟德尔模式。通过对第一代回交和第二代回交家系连续泌乳情况的分析，似乎所有杂种雌性都含有MMTV前病毒信息，这些信息可能在泌乳后期或在一定比例的后代小鼠中表达。这些综合结果与这些杂交中由多种因素调控的垂直传播基因组最为一致。
