从水疱性口炎病毒缺陷颗粒中分离出的RNA种类分析。

Analysis of the RNA species isolated from defective particles of vesicular stomatitis virus.

作者信息

Adler R, Banerjee A K

出版信息

J Gen Virol. 1976 Oct;33(1):51-60. doi: 10.1099/0022-1317-33-1-51.

Abstract

Serial high multiplicity passage of a cloned stock of vesicular stomatitis virus was found to generate defective interfering particles containing three size classes of RNA, with sedimentaiton coefficients of 31 S, 23 S and 19 S. The 31 S and 23 S RNA species were found to be complementary to both the 12 to 18 S and 31 S size classes of VSV mRNAs. The 19 S class of RNA was found to be partially base-paired. All three RNA species were found to contain ppAp at their 5' termini.

摘要

研究发现，对水泡性口炎病毒的一个克隆毒株进行连续的高倍数传代，会产生含有三种RNA大小类别的缺陷干扰颗粒，其沉降系数分别为31S、23S和19S。发现31S和23S RNA种类与VSV mRNA的12至18S和31S大小类别均互补。发现19S类RNA存在部分碱基配对。所有三种RNA种类在其5'末端均含有ppAp。