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从属于水疱性口炎病毒亚组的弹状病毒获得的RNA种类的寡核苷酸指纹图谱。

Oligonucleotide fingerprints of RNA species obtained from rhabdoviruses belonging to the vesicular stomatitis virus subgroup.

作者信息

Clewley J P, Bishop D H, Kang C Y, Coffin J, Schnitzlein W M, Reichmann M E, Shope R E

出版信息

J Virol. 1977 Jul;23(1):152-66. doi: 10.1128/JVI.23.1.152-166.1977.

DOI:10.1128/JVI.23.1.152-166.1977
PMID:196099
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC515811/
Abstract

The relationships among the genomes of various rhabdoviruses belonging to the vesicular stomatitis virus subgroup were analyzed by an oligonucleotide fingerprinting technique. Of 10 vesicular stomatitis viruses, Indiana serotype (VSV Indiana), obtained from various sources, either no, few, or many differences were observed in the oligonucleotide fingerprints of the 42S RNA species extracted from standard B virions. Analyses of the oligonucleotides obtained from RNA extracted from three separate preparations of VSV Indiana defective T particles showed that their RNAs contain fewer oligonucleotides than the corresponding B particle RNA species. The fingerprints of RNA obtained from five VSV New Jersey serotype viruses were easily distinguished from those of the VSV Indiana isolates. Three of the VSV New Jersey RNA fingerprints were similar to each other but quite different from those of the other two viruses. The RNA fingerprints of two Chandipura virus isolates (one obtained from India and one from Nigeria) were also unique, whereas the fingerprint of Cocal virus RNA was unlike that of the serologically related VSV Indiana.

摘要

采用寡核苷酸指纹技术分析了属于水疱性口炎病毒亚组的各种弹状病毒基因组之间的关系。从不同来源获得的10株水疱性口炎病毒,印第安纳血清型(VSV印第安纳),从标准B病毒粒子中提取的42S RNA种类的寡核苷酸指纹中,观察到无差异、少数差异或许多差异。对从VSV印第安纳缺陷T粒子的三个单独制剂中提取的RNA获得的寡核苷酸分析表明,它们的RNA比相应的B粒子RNA种类含有更少的寡核苷酸。从5株VSV新泽西血清型病毒中获得的RNA指纹很容易与VSV印第安纳分离株的指纹区分开来。其中3株VSV新泽西RNA指纹彼此相似,但与其他两种病毒的指纹有很大不同。两种钱德普拉病毒分离株(一株来自印度,一株来自尼日利亚)的RNA指纹也很独特,而科卡尔病毒RNA的指纹与血清学相关的VSV印第安纳不同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d4d/515811/29414d490ff1/jvirol00211-0173-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d4d/515811/d9c2e3acfd7a/jvirol00211-0166-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d4d/515811/b6acd18654c1/jvirol00211-0167-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d4d/515811/9bff4966803d/jvirol00211-0168-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d4d/515811/754eb429e23c/jvirol00211-0169-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d4d/515811/aab6d089443c/jvirol00211-0170-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d4d/515811/8f6c0d436666/jvirol00211-0172-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d4d/515811/29414d490ff1/jvirol00211-0173-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d4d/515811/d9c2e3acfd7a/jvirol00211-0166-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d4d/515811/b6acd18654c1/jvirol00211-0167-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d4d/515811/9bff4966803d/jvirol00211-0168-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d4d/515811/754eb429e23c/jvirol00211-0169-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d4d/515811/aab6d089443c/jvirol00211-0170-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d4d/515811/8f6c0d436666/jvirol00211-0172-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d4d/515811/29414d490ff1/jvirol00211-0173-a.jpg

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