He Feng, Yang Ying, Yang Guang, Yu Longjiang
Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
Z Naturforsch C J Biosci. 2008 Mar-Apr;63(3-4):181-8. doi: 10.1515/znc-2008-3-404.
A polysaccharide was isolated from the broth of cultured Streptomyces virginia H03 which was treated by ethanol deposition and savage method to remove the protein, and was purified using Sephadex G-150 column chromatography. The components of the polysaccharide were determined by gas chromatography. The purified polysaccharide was made up of mannose, glucose and galactose, in a 2:1:1 proportion. Its average apparent molecular weight was 3.76 x 10(4) Da which was determined by gel permeation chromatography. In addition, several antioxidant assays were adopted to investigate the antioxidant activity of the polysaccharide in vitro. The results indicated that the purified polysaccharide showed significant antioxidant activity against superoxide anion, hydrogen peroxide and 1,1-diphenyl-2-picrylhydrazyl radical, and lipid peroxidation as with standard antioxidants such as vitamin C. Furthermore, the polysaccharide had a better heat stability than vitamin C, which suggested that the polysaccharide might be a potent useful antioxidant.
从培养的维吉尼亚链霉菌H03发酵液中分离出一种多糖，通过乙醇沉淀和Savage法去除蛋白质，并用Sephadex G - 150柱色谱法进行纯化。采用气相色谱法测定多糖的组成成分。纯化后的多糖由甘露糖、葡萄糖和半乳糖组成，比例为2:1:1。通过凝胶渗透色谱法测定其平均表观分子量为3.76×10⁴Da。此外，采用多种抗氧化测定方法研究该多糖的体外抗氧化活性。结果表明，纯化后的多糖对超氧阴离子、过氧化氢和1,1 - 二苯基 - 2 - 苦基肼自由基以及脂质过氧化表现出显著的抗氧化活性，与维生素C等标准抗氧化剂相当。此外，该多糖比维生素C具有更好的热稳定性，这表明该多糖可能是一种有效的抗氧化剂。
