Engineering Research Center of Biotechnology for Active Substances, Ministry of Education; Chongqing Key Laboratory of Animal Biology, Chongqing Normal University, Chongqing, China.
School of Education, Chongqing Normal University, Chongqing, China.
J Food Biochem. 2019 Jul;43(7):e12899. doi: 10.1111/jfbc.12899. Epub 2019 May 20.
A neutral polysaccharide (DIP-1) from Duchesnea indica (Andr.) Focke was obtained by hot water extraction, ethanol precipitation and chromatographic separation (DEAE-52 cellulose anion-exchange column and Sephadex G-100 gel column). The physicochemical properties of DIP-1 were elucidated by gel permeation chromatography, monosaccharide composition, Fourier transform infrared spectrometry, UV-visible spectrophotometry, scanning electron microscope and Congo red test. The results indicated that DIP-1 was consisted of mannose, glucosamine, glucose, galactose and arabinose in a ratio of 1.00:0.42:18.36:14.17:0.81, and its molecular weight was 218.3 kDa. Meanwhile, DIP-1 presented a straight hexahedron structure, but no triple-helical conformation. In antioxidant activity tests, DIP-1 exhibited powerful scavenging activities on hydroxyl, DPPH, ABTS radicals and reducing power in a dose-dependent manner. Especially, DIP-1 demonstrated high inhibitory activities against SKOV-3 and Hep-G2 cells in vitro, with IC values of 1.42 and 1.23 mg/ml, respectively. PRACTICAL APPLICATIONS: D. indica has been used for a long time as a Chinese medicine for therapy of many diseases, including cancer, inflammation, leprosy, fever, bleeding and so on. At present, polysaccharides have attracted comprehensive attention because of a large range of pharmacological and biological properties, including antitumor, antidiabetic, antioxidant and immunomodulatory activity. In the present study, we purified and characterized a neutral polysaccharide from D. indica for the first time. Moreover, the neutral polysaccharide exhibits significant antioxidant and antitumor activities. Therefore, the present study laid a foundation for the high-value application of D. indica polysaccharides in functional food and pharmaceutical industries.
从蓬蘽（Duchesnea indica (Andr.) Focke）中提取的中性多糖（DIP-1）采用热水提取、乙醇沉淀和色谱分离（DEAE-52 纤维素阴离子交换柱和 Sephadex G-100 凝胶柱）得到。通过凝胶渗透色谱、单糖组成、傅里叶变换红外光谱、紫外可见分光光度法、扫描电子显微镜和刚果红试验阐明了 DIP-1 的理化性质。结果表明，DIP-1 由甘露糖、氨基葡萄糖、葡萄糖、半乳糖和阿拉伯糖以 1.00:0.42:18.36:14.17:0.81 的比例组成，其分子量为 218.3 kDa。同时，DIP-1 呈现出直六面体结构，但没有三螺旋构象。在抗氧化活性测试中，DIP-1 对羟基、DPPH、ABTS 自由基和还原能力表现出强大的清除活性，呈剂量依赖性。特别是，DIP-1 在体外对 SKOV-3 和 Hep-G2 细胞表现出高抑制活性，IC 值分别为 1.42 和 1.23 mg/ml。实际应用：蓬蘽长期以来一直被用作治疗多种疾病的中药，包括癌症、炎症、麻风病、发热、出血等。目前，由于具有广泛的药理和生物学特性，包括抗肿瘤、抗糖尿病、抗氧化和免疫调节活性，多糖已引起广泛关注。在本研究中，我们首次从蓬蘽中纯化和表征了一种中性多糖。此外，中性多糖表现出显著的抗氧化和抗肿瘤活性。因此，本研究为蓬蘽多糖在功能食品和制药工业中的高值应用奠定了基础。
