Ishida Mitsuaki, Kushima Ryoji, Okabe Hidetoshi
Department of Clinical Laboratory Medicine, Shiga University of Medical Science, Shiga, Japan.
J Cutan Pathol. 2008 Oct;35(10):926-30. doi: 10.1111/j.1600-0560.2007.00936.x. Epub 2008 Jun 5.
Recently, immunoreactivity to D2-40, a monoclonal antibody to lymphatic endothelium, was shown in a subgroup of epidermal basal cells and the majority of squamous cell carcinomas, but the immunoreactivity to this antibody has not been examined in basal cell carcinomas (BCCs) of the skin.
Expression of D2-40 was analyzed together with that of cytokeratin (CK) 17, CK 19, CD34 and Ber-EP4 by immunohistochemical methods in 10 non-neoplastic skin tissue samples and 20 BCCs.
Immunoreactivity to D2-40 was shown in the basal cells at the outer root sheath (ORS) of hair follicles, similar to the CK 19 expression pattern. CK 17 was strongly expressed in the suprabasal cells at the ORS. D2-40 and CK 19 were focally expressed in 13/20 (65%) and 14/20 (70%) cases of BCCs, respectively, although the distributions of D2-40 and CK 19 immunoreactivity were not always identical. However, BCC cells were constantly positive for CK 17 even tumor cells that were positive for D2-40 and/or CK 19. Ber-EP4 was diffusely expressed in all BCCs, and CD34 was focally expressed in 2/20 cases.
Our results suggested that D2-40 immunoreactivity in BCCs showed differentiation toward the ORS of hair follicles.
最近，在一部分表皮基底细胞和大多数鳞状细胞癌中发现了对淋巴管内皮细胞单克隆抗体D2-40的免疫反应性，但尚未对皮肤基底细胞癌（BCC）中该抗体的免疫反应性进行研究。
采用免疫组织化学方法，对10份非肿瘤性皮肤组织样本和20例BCC中D2-40的表达与细胞角蛋白（CK）17、CK 19、CD34和Ber-EP4的表达进行分析。
毛囊外根鞘（ORS）的基底细胞显示出对D2-40的免疫反应性，类似于CK 19的表达模式。CK 17在ORS的基底上层细胞中强烈表达。D2-40和CK 19分别在13/20（65%）和14/20（70%）的BCC病例中呈局灶性表达，尽管D2-40和CK 19免疫反应性的分布并不总是相同。然而，即使是对D2-40和/或CK 19呈阳性的肿瘤细胞，BCC细胞对CK 17也始终呈阳性。Ber-EP4在所有BCC中均呈弥漫性表达，CD34在2/20例中呈局灶性表达。
我们的结果表明，BCC中D2-40免疫反应性显示出向毛囊ORS的分化。
