Schwartz B S, Ford D P, Childs J E, Rothman N, Thomas R J
Department of Environmental Health Sciences, Johns Hopkins University School of Hygiene and Public Health, Baltimore, MD 21205.
Am J Epidemiol. 1991 Jul 1;134(1):86-95. doi: 10.1093/oxfordjournals.aje.a115996.
Anti-tick saliva antibody (ATSA) has potential as a biologic marker of exposure to tick bites. In 1989, we conducted a cross-sectional study of 304 outdoor workers in Monmouth County, New Jersey, to evaluate associations between self-reported tick exposure, ATSA status, and Lyme disease antibody status. ATSA levels 1) were correlated with an index of tick exposure on the basis of three self-reported measures of tick exposure and outdoor hours worked per week (p = 0.01); 2) were consistently higher in pet owners compared with persons without pets (p = 0.03); and 3) when examined by duration since last tick bite, peaked at 3-5 weeks after tick bite and then declined (p = 0.06). ATSA levels dichotomized at the 75th percentile (approximately two standard deviations above the mean ATSA optical density of 25 subjects who denied recent tick exposure) were associated with self-reported tick exposure: adjusted odds ratios for high ATSA levels were 4.2 (95% confidence interval (CI) 0.9-18.9) for moderate (versus none) tick exposure and 5.8 (95% CI 1.2-27.2) for high (versus none) tick exposure. Finally, high ATSA levels were associated with Lyme disease seropositivity, with an adjusted odds ratio of 3.2 (95% CI 1.3-7.6). The data suggest that ATSA is a biologic marker of tick exposure that is a risk factor for Lyme disease seropositivity.
抗蜱唾液抗体（ATSA）有潜力作为蜱叮咬暴露的生物标志物。1989年，我们对新泽西州蒙茅斯县的304名户外工作者进行了一项横断面研究，以评估自我报告的蜱暴露、ATSA状态和莱姆病抗体状态之间的关联。ATSA水平：1）基于三种自我报告的蜱暴露测量指标和每周户外工作时长，与蜱暴露指数相关（p = 0.01）；2）宠物主人的ATSA水平始终高于无宠物者（p = 0.03）；3）按自上次蜱叮咬后的持续时间进行检查时，ATSA水平在蜱叮咬后3 - 5周达到峰值，然后下降（p = 0.06）。将ATSA水平在第75百分位数处进行二分法划分（大约比25名否认近期有蜱暴露的受试者的平均ATSA光密度高两个标准差）与自我报告的蜱暴露相关：中度（相对于无）蜱暴露时，高ATSA水平的调整优势比为4.2（95%置信区间（CI）0.9 - 18.9），高度（相对于无）蜱暴露时为5.8（95% CI 1.2 - 27.2）。最后，高ATSA水平与莱姆病血清阳性相关，调整优势比为3.2（95% CI 1.3 - 7.6）。数据表明，ATSA是蜱暴露的生物标志物，也是莱姆病血清阳性的危险因素。
