Wiesner G, Schiewe-Langgartner F, Lindner R, Gruber M
Department of Anaesthesiology, German Heart Centre Munich, Germany.
Anaesthesia. 2008 Aug;63(8):861-4. doi: 10.1111/j.1365-2044.2008.05498.x. Epub 2008 Jun 6.
We have assessed, for the first time, genotoxicity (i.e. sister chromatid exchanges and micronuclei) in anaesthetists exposed to a single volatile anaesthetic (sevoflurane) without nitrous oxide. The anaesthetists were exposed to an 8-h time-weighted average of 0.2 parts per million sevoflurane. Internists served as non-exposed controls. Mean (SD) sister chromatid exchanges per cell were significantly higher in anaesthetists compared to internists (6.6 (0.9) vs 5.1 (0.8); p < 0.001) whereas median (IQR [range]) micronuclei per 1000 binucleated cells did not differ (9.5 (6.3-10.8 [2.0-15.5]) vs 8.5 (6.0-10.5 [3.0-25.5]), respectively). Although the anaesthetists were exposed to rather low concentrations of sevoflurane, this 30% increase of sister chromatid exchanges is in agreement with a recently reported 300% increase with a high level exposure to sevoflurane and nitrous oxide. Omitting nitrous oxide does not normalise increased rates of sister chromatid exchanges.
我们首次评估了仅接触单一挥发性麻醉剂（七氟醚）而未接触氧化亚氮的麻醉医生的遗传毒性（即姐妹染色单体交换和微核）。这些麻醉医生接触七氟醚的8小时时间加权平均浓度为百万分之0.2。内科医生作为未接触的对照组。与内科医生相比，麻醉医生每个细胞的平均（标准差）姐妹染色单体交换显著更高（6.6（0.9）对5.1（0.8）；p<0.001），而每1000个双核细胞的中位（四分位间距[范围]）微核无差异（分别为9.5（6.3 - 10.8[2.0 - 15.5]）对8.5（6.0 - 10.5[3.0 - 25.5]））。尽管麻醉医生接触的七氟醚浓度相当低，但姐妹染色单体交换增加30%与最近报道的高浓度接触七氟醚和氧化亚氮时增加300%是一致的。不使用氧化亚氮并不能使姐妹染色单体交换率升高的情况恢复正常。
