Verit Fatma Ferda, Erel Ozcan, Celik Hakim
Department of Obstetrics and Gynecology, Harran University, Sanliurfa, Turkey.
Redox Rep. 2008;13(3):134-8. doi: 10.1179/135100008X259259.
There is increasing evidence that oxidative stress may play a role in the pathophysiology of hyperemesis gravidarum. Serum paraoxonase-1 (PON-1) is a high density lipoprotein (HDL)-associated enzyme that prevents oxidative modification of low density lipoprotein. The aim of the study was to measure the serum levels of PON-1 activity in women with hyperemesis gravidarum. Thirty-four women with hyperemesis gravidarum and 31 healthy pregnant women were enrolled in the study. Serum PON-1 activity was measured spectrophotometrically. Lipid hydroperoxide (LOOH) levels were measured by iodometric assay. PON-1 activity was significantly lower and LOOH levels were significantly higher in pregnant women with hyperemesis gravidarum than in healthy pregnant women (P < 0.0001, for all). There were significant correlations between PON-1 and LOOH, triglyceride, total cholesterol, HDL, low density lipoprotein (LDL) and high sensitive C-reactive protein (HSCRP; P < 0.0001, for all). By using multiple regression analysis LDL, HDL, HSCRP and LOOH were independent determinants of serum PON-1 activity in the study. Decreased PON-1 activity might be related to increased oxidative stress and inflammation in pregnant women with hyperemesis gravidarum. Subjects with hyperemesis gravidarum might be more prone to the development of atherogenesis due to low serum PON-1 activity.
越来越多的证据表明，氧化应激可能在妊娠剧吐的病理生理学中起作用。血清对氧磷酶-1（PON-1）是一种与高密度脂蛋白（HDL）相关的酶，可防止低密度脂蛋白的氧化修饰。本研究的目的是测量妊娠剧吐女性的血清PON-1活性水平。34名妊娠剧吐女性和31名健康孕妇参与了该研究。采用分光光度法测量血清PON-1活性。通过碘量法测量脂质过氧化氢（LOOH）水平。妊娠剧吐孕妇的PON-1活性显著低于健康孕妇，而LOOH水平显著高于健康孕妇（所有P<0.0001）。PON-1与LOOH、甘油三酯、总胆固醇、HDL、低密度脂蛋白（LDL）和高敏C反应蛋白（HSCRP）之间存在显著相关性（所有P<0.0001）。通过多元回归分析，LDL、HDL、HSCRP和LOOH是本研究中血清PON-1活性的独立决定因素。PON-1活性降低可能与妊娠剧吐孕妇氧化应激和炎症增加有关。由于血清PON-1活性低，妊娠剧吐患者可能更容易发生动脉粥样硬化。
