Rivero Francisco
The Hull York Medical School and Department of Biological Sciences, University of Hull, Hull HU6 7RX, United Kingdom.
Int Rev Cell Mol Biol. 2008;267:343-97. doi: 10.1016/S1937-6448(08)00633-3.
Endocytosis, an essential process of all eukaryotic cells, requires the actin cytoskeleton for proper functioning. The soil amoeba Dictyostelium discoideum is well known for its contribution to the actin cytoskeleton field. The genetic tractability and the availability of appropriate tools have made of Dictyostelium an attractive model for studies of endocytosis and vesicle trafficking as well. These tools include a large palette of fluorescent protein fusions and the combination of improved fractionation methods with high throughput techniques along with the recently propagated use of the amoeba a host for microbial pathogens. In this review I discuss in a comprehensive manner the evidence accumulated in the literature towards a participation of components of the microfilament system of D. discoideum in endocytic trafficking and conclude with a model that describes the sequence of events and the components involved during the well-investigated uptake phase of the endocytic process in the soil amoeba.
内吞作用是所有真核细胞的一个基本过程，其正常运作需要肌动蛋白细胞骨架。土壤变形虫盘基网柄菌因其对肌动蛋白细胞骨架领域的贡献而闻名。其遗传易处理性和合适工具的可用性使盘基网柄菌成为研究内吞作用和囊泡运输的一个有吸引力的模型。这些工具包括大量荧光蛋白融合体，以及改进的分级分离方法与高通量技术的结合，还有最近将变形虫作为微生物病原体宿主的广泛应用。在这篇综述中，我全面讨论了文献中积累的关于盘基网柄菌微丝系统成分参与内吞运输的证据，并以一个模型作为结论，该模型描述了土壤变形虫内吞过程中经过充分研究的摄取阶段所涉及的事件顺序和成分。
