Kepshire Dax S, Davis Scott C, Dehghani Hamid, Paulsen Keith D, Pogue Brian W
Thayer School of Engineering, Dartmouth College, New Hampshire 03755, USA.
Appl Opt. 2007 Apr 1;46(10):1669-78. doi: 10.1364/ao.46.001669.
Subsurface tomography with diffuse light has been investigated with a noncontact approach to characterize the performance of absorption and fluorescence imaging. Using both simulations and experiments, the reconstruction of local subsurface heterogeneity is demonstrated, but the recovery of target size and fluorophore concentration is not linear when changes in depth occur, whereas the mean position of the object for experimental fluorescent and absorber targets is accurate to within 0.5 and 1.45 mm when located within the first 10 mm below the surface. Improvements in the linearity of the response with depth appear to remain challenging and may ultimately limit the approach to detection rather than characterization applications. However, increases in tissue curvature and/or the addition of prior information are expected to improve the linearity of the response. The potential for this type of imaging technique to serve as a surgical guide is highlighted.
已采用非接触方法对漫射光的表面下断层扫描进行了研究，以表征吸收成像和荧光成像的性能。通过模拟和实验，展示了局部表面下异质性的重建，但当深度发生变化时，目标尺寸和荧光团浓度的恢复是非线性的，而当实验性荧光和吸收体目标位于表面以下前10毫米内时，其平均位置的精确到0.5毫米和1.45毫米以内。响应随深度的线性度的改善似乎仍然具有挑战性，并且最终可能会限制该方法在检测而非表征应用中的使用。然而，组织曲率的增加和/或先验信息的添加有望改善响应的线性度。突出了这种成像技术作为手术指南的潜力。
