Silva Paulo Roberto da, Rabelo Rosiley Aparecida de Souza, Terra Ana Paula Sarreta, Teixeira David Nascimento Silva
Departamento de Ciências Biológicas, Universidade Federal do Triângulo Mineiro, Uberaba, M.G.
Rev Soc Bras Med Trop. 2008 Mar-Apr;41(2):158-62. doi: 10.1590/s0037-86822008000200005.
This study identified Cryptococcus neoformans varieties isolated from 35 patients at teaching hospital of the Federal University of the Triângulo Mineiro and evaluated the susceptibility to antifungal agents among these samples using the protocol M27-A2 from the National Committee for Clinical Laboratory Standards. The gattii variety was identified in 11.4% of the cases (n = 4). The minimum inhibitory concentration (mg/ml) of Cryptococcus neoformans neoformans isolates ranged from 0.062 to 2.000 (amphotericin B), 0.250 to 8.000 (fluconazole), 0.062 to 1.000 (itraconazole) and 0.125 to 1.000 (ketoconazole). The gattii variety presented a minimum inhibitory concentration range of 0.125 to 2.000 (amphotericin B), 0.250 to 16.00 (fluconazole), 0.062 to 1.000 (itraconazole) and 0.125 to 4.000 (ketoconazole). Two isolates resistant to itraconazole and two resistant to amphotericin B (one isolate of each variety per antifungal agent) were found. These data show the importance of determining the variety and minimum inhibitory concentration of Cryptococcus neoformans isolates, in order to monitor resistance development and enable better treatment for cryptococcosis.
本研究鉴定了从米纳斯三角联邦大学教学医院的35例患者中分离出的新型隐球菌变种，并使用美国国家临床实验室标准委员会的M27 - A2方案评估了这些样本中抗真菌药物的敏感性。在11.4%的病例（n = 4）中鉴定出了加蒂变种。新型隐球菌新生变种分离株的最低抑菌浓度（mg/ml）范围为0.062至2.000（两性霉素B）、0.250至8.000（氟康唑）、0.062至1.000（伊曲康唑）和0.125至1.000（酮康唑）。加蒂变种的最低抑菌浓度范围为0.125至2.000（两性霉素B）、0.250至16.00（氟康唑）、0.062至1.000（伊曲康唑）和0.125至4.000（酮康唑）。发现了两株对伊曲康唑耐药的分离株和两株对两性霉素B耐药的分离株（每种抗真菌药物各有一个变种的分离株）。这些数据表明确定新型隐球菌分离株的变种和最低抑菌浓度的重要性，以便监测耐药性的发展并为隐球菌病提供更好的治疗。
