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利用聚合酶链反应扩增的16S rRNA特异性DNA对趋磁水生螺菌进行系统发育分析。

Phylogenetic analysis of Aquaspirillum magnetotacticum using polymerase chain reaction-amplified 16S rRNA-specific DNA.

作者信息

Eden P A, Schmidt T M, Blakemore R P, Pace N R

机构信息

Department of Microbiology, University of New Hampshire, Durham 03824.

出版信息

Int J Syst Bacteriol. 1991 Apr;41(2):324-5. doi: 10.1099/00207713-41-2-324.

DOI:10.1099/00207713-41-2-324
PMID:1854644
Abstract

The 16S rRNA gene of the magnetotactic magnetogen Aquaspirillum magnetotacticum MS1 was amplified by a polymerase chain reaction, using two eubacterial consensus oligodeoxynucleotide primers flanking the majority of the 16S rRNA gene, cloned, and sequenced. Phylogenetic analysis revealed that A. magnetotacticum MS1 belongs to the alpha-group of proteobacteria. This assignment offers perspective on the biochemical properties of A. magnetotacticum, since this organism is expected to have the general properties that are common to this phylogenetic group.

摘要

利用聚合酶链反应扩增趋磁细菌趋磁水生螺菌MS1的16S rRNA基因,使用位于16S rRNA基因大部分区域两侧的两条真细菌共有寡脱氧核苷酸引物,进行克隆和测序。系统发育分析表明,趋磁水生螺菌MS1属于变形菌门的α亚群。这一分类为趋磁水生螺菌的生化特性提供了研究视角,因为预计该生物体具有这一系统发育组共有的一般特性。

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