Redburn A C, Patel B K
Faculty of Science and Technology, Griffith University, Nathan, Brisbane, Queensland, Australia.
FEMS Microbiol Lett. 1993 Oct 1;113(1):81-6. doi: 10.1111/j.1574-6968.1993.tb06492.x.
The 16S rRNA gene of the thermophilic sulfate-reducing bacterium Desulfotomaculum thermobenzoicum was amplified by polymerase chain reaction using two eubacterial consensus oligodeoxynucleotide primers flanking the majority of the 16S rRNA gene, cloned, and sequenced. Phylogenetic analysis revealed that D. thermobenzoicum belongs to the Gram-positive (low G + C content) branch and is more related to the thermophilic sulfate-reducing bacterium, D. australicum than the moderate thermophile D. nigrificans, or the mesophiles D. orientis, and D. ruminis. This relationship is further strengthened by the presence of an unusual idiosyncrasy in helix 6 of the 16S rRNA gene of D. thermobenzoicum resembling that of D. australicum but not found in other desulfotomacula species and in any other bacteria sequenced to date.
利用位于16S rRNA基因大部分区域两侧的两个真细菌共有寡脱氧核苷酸引物，通过聚合酶链反应扩增嗜热硫酸盐还原菌热苯甲酸脱硫肠状菌的16S rRNA基因，进行克隆并测序。系统发育分析表明，热苯甲酸脱硫肠状菌属于革兰氏阳性（低G + C含量）分支，与嗜热硫酸盐还原菌澳大利亚脱硫肠状菌的关系比中度嗜热菌黑化脱硫肠状菌或嗜温菌东方脱硫肠状菌和瘤胃脱硫肠状菌更为密切。热苯甲酸脱硫肠状菌16S rRNA基因螺旋6中存在一种不寻常的特性，类似于澳大利亚脱硫肠状菌，但在其他脱硫肠状菌物种和迄今为止测序的任何其他细菌中均未发现，这进一步加强了这种关系。
