Microbiology Department, College of Veterinary Medicine, University of Basrah, Basrah, Iraq.
Department of Public Health, College of Veterinary Medicine, University of Basrah, Basrah, Iraq.
Open Vet J. 2024 Mar;14(3):779-786. doi: 10.5455/OVJ.2024.v14.i3.4. Epub 2024 Mar 31.
Milk-borne bacteria cause degradation of milk products and constitute a significant risk to public health.
The objectives of the present study are to determine the microbiological quality of dairy products and to investigate pathogenic microorganisms.
A total of 60 samples of raw milk, homemade cheese, and yogurt were randomly selected from different retail marketplaces in Basrah. The bacteriological and biochemical tests were utilized to identify the pathogens in dairy samples, as well as the molecular technique was used as an accurate diagnostic test.
The prevalence of contamination of milk products with various isolates was estimated as 50% (95% Cl: 36.8-63.2). The mean of total bacteria count for cheese was 7.29 ± 2.70, raw milk 4.62 ± 2.86, and yogurt 2.87 ± 1.05, with a significant -value ( = 0.001). The mean count of aerobic spore-forming (ASF) contaminated raw milk was analyzed as 3.77 ± 1.18 and less contamination detected in the yogurt samples with mean of ASF was estimated as 2.52 ± 1.47 SD log 10 CFU/ml. A range of important microorganisms to human health were identified by employing the VITEK_2 system and sequencing 16S rDNA gene, including , , , and
The study indicates that there is a high level of bacterial contamination in dairy products with different bacteria species, which is medically important. Therefore, food safety management must be implemented to reduce biological risks carried by dairy products and ensure healthy food for consumers.
牛奶中的细菌会导致奶制品变质，对公众健康构成重大威胁。
本研究旨在确定乳制品的微生物质量并调查致病菌。
从巴士拉不同零售市场随机抽取 60 份生奶、自制奶酪和酸奶样本。利用细菌学和生化试验对乳制品样本中的病原体进行鉴定，并采用分子技术作为准确的诊断试验。
奶制品中各种分离物的污染率估计为 50%（95%置信区间：36.8-63.2）。奶酪的总细菌数平均值为 7.29 ± 2.70，生奶为 4.62 ± 2.86，酸奶为 2.87 ± 1.05，差异有统计学意义（ = 0.001）。污染的需氧芽孢形成菌（ASF）在生奶中的平均计数为 3.77 ± 1.18，酸奶样本中的 ASF 污染程度较低，平均计数为 2.52 ± 1.47 SD log 10 CFU/ml。通过 VITEK_2 系统和 16S rDNA 基因测序鉴定出了对人类健康很重要的一系列微生物，包括 、 、 、 。
研究表明，不同种类的奶制品中存在高水平的细菌污染，这具有医学重要性。因此，必须实施食品安全管理，以降低奶制品携带的生物风险，确保消费者食用健康食品。
