Antonio Martin, Dada-Adegbola Hannah, Biney Ekow, Awine Tim, O'Callaghan John, Pfluger Valentin, Enwere Godwin, Okoko Brown, Oluwalana Claire, Vaughan Adeola, Zaman Syed Ma, Pluschke Gerd, Greenwood Brian M, Cutts Felicity, Adegbola Richard A
Medical Research Council Laboratories, Banjul, The Gambia.
BMC Infect Dis. 2008 Jun 11;8:81. doi: 10.1186/1471-2334-8-81.
The study describes the molecular epidemiology of Streptococcus pneumoniae causing invasive disease in Gambian children
One hundred and thirty-two S. pneumoniae isolates were recovered from children aged 2-29 months during the course of a pneumococcal conjugate vaccine trial conducted in The Gambia of which 131 were characterized by serotyping, antibiotic susceptibility, BOX-PCR and MLST.
Twenty-nine different serotypes were identified; serotypes 14, 19A, 12F, 5, 23F, and 1 were common and accounted for 58.3% of all serotypes overall. MLST analysis showed 72 sequence types (STs) of which 46 are novel. eBURST analysis using the stringent 6/7 identical loci definition, grouped the isolates into 17 clonal complexes and 32 singletons. The population structure of the 8 serotype 1 isolates obtained from 4 vaccinated and 2 unvaccinated children were the same (ST 618) except that one (ST3336) of the isolates from an unvaccinated child had a novel ST which is a single locus variant of ST 618.
We provide the first background data on the genetic structure of S. pneumoniae causing IPD prior to PC7V use in The Gambia. This data will be important for assessing the impact of PC7V in post-vaccine surveillance from The Gambia.
本研究描述了冈比亚儿童侵袭性疾病肺炎链球菌的分子流行病学。
在冈比亚进行的肺炎球菌结合疫苗试验过程中，从2至29个月大的儿童中分离出132株肺炎链球菌，其中131株通过血清分型、抗生素敏感性、BOX-PCR和多位点序列分型进行了特征分析。
鉴定出29种不同血清型；14、19A、12F、5、23F和1型血清型较为常见，占所有血清型总数的58.3%。多位点序列分型分析显示72种序列型（STs），其中46种是新的。使用严格的6/7个相同位点定义进行的eBURST分析将分离株分为17个克隆复合体和32个单株。从4名接种疫苗和2名未接种疫苗的儿童中获得的8株1型血清型分离株的群体结构相同（ST 618），只是从未接种疫苗儿童中分离出的一株（ST3336）具有新的ST，它是ST 618的单一位点变体。
我们提供了冈比亚在使用PC7V之前引起侵袭性肺炎球菌病的肺炎链球菌遗传结构的首批背景数据。这些数据对于评估PC7V在冈比亚疫苗接种后监测中的影响将具有重要意义。
