Biju Vasudevanpillai, Itoh Tamitake, Anas Abdulaziz, Sujith Athiyanathil, Ishikawa Mitsuru
Nano-Bioanalysis Team, Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14 Hayashi-cho, Takamatsu, Kagawa, 761-0395, Japan.
Anal Bioanal Chem. 2008 Aug;391(7):2469-95. doi: 10.1007/s00216-008-2185-7. Epub 2008 Jun 12.
We review the syntheses, optical properties, and biological applications of cadmium selenide (CdSe) and cadmium selenide-zinc sulfide (CdSe-ZnS) quantum dots (QDs) and gold (Au) and silver (Ag) nanoparticles (NPs). Specifically, we selected the syntheses of QDs and Au and Ag NPs in aqueous and organic phases, size- and shape-dependent photoluminescence (PL) of QDs and plasmon of metal NPs, and their bioimaging applications. The PL properties of QDs are discussed with reference to their band gap structure and various electronic transitions, relations of PL and photoactivated PL with surface defects, and blinking of single QDs. Optical properties of Ag and Au NPs are discussed with reference to their size- and shape-dependent surface plasmon bands, electron dynamics and relaxation, and surface-enhanced Raman scattering (SERS). The bioimaging applications are discussed with reference to in vitro and in vivo imaging of live cells, and in vivo imaging of cancers, tumor vasculature, and lymph nodes. Other aspects of the review are in vivo deep tissue imaging, multiphoton excitation, NIR fluorescence and SERS imaging, and toxic effects of NPs and their clearance from the body.
我们综述了硒化镉（CdSe）、硒化镉-硫化锌（CdSe-ZnS）量子点（QD）以及金（Au）和银（Ag）纳米颗粒（NP）的合成、光学性质及生物应用。具体而言，我们选取了水相和有机相中量子点以及金和银纳米颗粒的合成、量子点尺寸和形状依赖的光致发光（PL）及金属纳米颗粒的等离子体激元，以及它们的生物成像应用。结合量子点的带隙结构和各种电子跃迁、PL及光活化PL与表面缺陷的关系以及单个量子点的闪烁现象，讨论了量子点的PL性质。结合银和金纳米颗粒尺寸和形状依赖的表面等离子体带、电子动力学和弛豫以及表面增强拉曼散射（SERS），讨论了它们的光学性质。结合活细胞的体外和体内成像以及癌症、肿瘤脉管系统和淋巴结的体内成像，讨论了生物成像应用。综述的其他方面包括体内深部组织成像、多光子激发、近红外荧光和SERS成像，以及纳米颗粒的毒性作用及其从体内的清除。
