El-Gendy Mervat M A, Shaaban M, El-Bondkly A M, Shaaban K A
Department of Chemistry of Natural and Microbial Products, Division of Pharmaceutical Industries, National Research Centre, El-Behoos st. 33, Dokki-Cairo, Egypt.
Appl Biochem Biotechnol. 2008 Jul;150(1):85-96. doi: 10.1007/s12010-008-8192-5. Epub 2008 Jun 13.
In our searching program for bioactive secondary metabolites from marine Streptomycetes, three microbial benzopyrone derivatives (1-3), 7-methylcoumarin (1) and two flavonoides, rhamnazin (2) and cirsimaritin (3), were obtained during the working up of the ethyl acetate fraction of a marine Streptomyces fusant obtained from protoplast fusion between Streptomyces strains Merv 1996 and Merv 7409. The structures of the three compounds (1-3) were established by nuclear magnetic resonance, mass, UV spectra, and by comparison with literature data. Marine Streptomyces strains were identified based on their phenotypic and chemotypic characteristics as two different bioactive strains of the genus Streptomyces. We described here the fermentation, isolation, as well as the biological activity of these bioactive compounds. The isolated compounds (1-3) are reported here as microbial products for the first time.
在我们从海洋链霉菌中寻找生物活性次生代谢产物的研究项目中，在对通过链霉菌菌株Merv 1996和Merv 7409原生质体融合获得的海洋链霉菌融合体的乙酸乙酯部分进行后处理过程中，得到了三种微生物苯并吡喃酮衍生物（1 - 3）、7 - 甲基香豆素（1）以及两种黄酮类化合物，鼠李素（2）和cirsimaritin（3）。通过核磁共振、质谱、紫外光谱以及与文献数据对比确定了这三种化合物（1 - 3）的结构。基于其表型和化学型特征，海洋链霉菌菌株被鉴定为链霉菌属的两种不同生物活性菌株。我们在此描述了这些生物活性化合物的发酵、分离以及生物活性。这里首次报道分离得到的化合物（1 - 3）为微生物产物。
